Biochemistry Laboratory, Department of Crop Improvement, College of Agriculture, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur, India.
Int J Food Sci Nutr. 2013 Mar;64(2):248-59. doi: 10.3109/09637486.2012.713918. Epub 2012 Aug 6.
RNA interference (RNAi) is a homology-dependent gene-silencing technology that involves double-stranded RNA directed against a target gene. This technique has emerged as powerful tool in understanding the functions of a number of genes in recent years. For the improvement in the nutritional status of the plants and reduction in the level of antinutrients, the conventional breeding methods were not completely successful in achieving the tissue-specific regulation of some genes. RNAi has shown successful results in a number of plant species for nutritional improvement, change in morphology and alteration in metabolite synthesis. This technology has been applied mostly in genetic engineering of important crop plants, and till date there are no reports of its application for the improvement of traditional/underutilized crops. In this study, we discuss current knowledge of RNAi function and concept and strategies for the improvement of traditional crops. Practical application. Although RNAi has been extensively used for the improvement of popular crops, no attention has been given for the use of this technology for the improvement of underutilized crops. This study describes the importance of use of this technology for the improvement of underutilized crops.
RNA 干扰 (RNAi) 是一种同源依赖性基因沉默技术，涉及双链 RNA 靶向靶基因。近年来，该技术已成为研究许多基因功能的有力工具。为了改善植物的营养状况和降低抗营养物质水平，传统的育种方法在实现某些基因的组织特异性调控方面并不完全成功。RNAi 在许多植物物种的营养改善、形态改变和代谢产物合成改变方面取得了成功的结果。该技术主要应用于重要作物的遗传工程，迄今为止，尚无关于其在传统/低利用作物改良中的应用的报道。在这项研究中，我们讨论了 RNAi 功能的当前知识以及传统作物改良的概念和策略。实际应用。尽管 RNAi 已被广泛用于改良受欢迎的作物，但尚未有人关注将该技术用于改良低利用作物。本研究描述了该技术在改良低利用作物中的重要性。
