Matsuoka R, Mori H, Tomonari R, Tomonari M
Department of Obstetrics and Gynecology, Hitachi General Hospital, Ibaraki, Japan.
Nihon Naibunpi Gakkai Zasshi. 1990 Nov 20;66(11):1127-37. doi: 10.1507/endocrine1927.66.11_1127.
The circadian and pulsatile secretions of prolactin were studied in 15 pregnant and 5 puerperal women. Blood samples were taken every 15 minutes for 3 hours during daytime and at night. Diurnal levels of prolactin and estradiol were examined in 135 pregnant women. In addition, prolactin and estradiol of 8 parturient women were measured every 15 minutes until 24 hours after delivery. Prolactin and estradiol levels increased gradually in advance of pregnancy, and a positive correlation was revealed between prolactin(Y) and gestational weeks(X): Y = 7.125X-42.997, r = 0.9955. Furthermore, a positive correlation between prolactin(Y) and estradiol(X) levels was observed: Y = 4.439X + 44.115, r = 0.7590. The circadian secretion of prolactin was observed in 15 out of 20 cases studied. The pulsatile secretion of prolactin was revealed in all 20 cases. The daytime pulse-frequency significantly decreased in the 2nd trimester and that of the nighttime also decreased in the 3rd trimester. The pulse-amplitude increased in advance of pregnancy, but amplitude/baseline value ratio was stationary 28% during the daytime and 34% during the nighttime in every trimester of pregnancy. 63.0% of coincidence in prolactin pulse with increase of immunoreactive LH-hCG was observed. The pulsatile secretion of hCG has not been yet reported. However, the pulsatile increase of immunoreactive LH-hCG was observed in 10 pregnancies, while LH was not detected in any pregnancy by highly specific LH assay. This suggested that immunoreactive hCG changed in a pulsatile manner in the blood of pregnant subjects. A highly significant decline of prolactin levels was observed from 10 hours before until 4 hours after delivery, which did not correlate with changes of estradiol levels. These results indicated that the pulsatility and circadian secretion of prolactin were preserved during pregnancy and puerperium and also suggested that prolactin secretion during pregnancy, delivery and puerperium was regulated by at least 3 factors: estradiol, dopamine and LH-hCG.
对15名孕妇和5名产妇的催乳素昼夜节律性和脉冲式分泌进行了研究。白天和夜间每15分钟采集一次血样，共采集3小时。检测了135名孕妇催乳素和雌二醇的日间水平。此外，对8名产妇产后24小时内每15分钟测量一次催乳素和雌二醇。妊娠前催乳素和雌二醇水平逐渐升高，催乳素（Y）与孕周（X）呈正相关：Y = 7.125X - 42.997，r = 0.9955。此外，还观察到催乳素（Y）与雌二醇（X）水平呈正相关：Y = 4.439X + 44.115，r = 0.7590。在所研究的20例中有15例观察到催乳素的昼夜分泌。所有20例均显示出催乳素的脉冲式分泌。孕中期白天的脉冲频率显著降低，孕晚期夜间的脉冲频率也降低。脉冲幅度在妊娠前升高，但妊娠各期白天的幅度/基线值比稳定在28%，夜间为34%。观察到63.0%的催乳素脉冲与免疫反应性LH - hCG升高同时出现。尚未报道hCG的脉冲式分泌。然而，在10例妊娠中观察到免疫反应性LH - hCG的脉冲式升高，而通过高特异性LH检测在任何妊娠中均未检测到LH。这表明妊娠受试者血液中免疫反应性hCG以脉冲方式变化。从分娩前10小时到分娩后4小时观察到催乳素水平显著下降，这与雌二醇水平的变化无关。这些结果表明，妊娠和产褥期催乳素的脉冲性和昼夜分泌得以保留，也提示妊娠、分娩和产褥期催乳素的分泌至少受3种因素调节：雌二醇、多巴胺和LH - hCG。
