Laboratoire de Physiologie et Ecologie Microbienne, Université Libre de Bruxelles, c/o ISP, 642 Rue Engeland, B-1180 Brussels, Belgium.
Fungal Biol. 2012 Aug;116(8):883-9. doi: 10.1016/j.funbio.2012.05.005. Epub 2012 May 29.
The white-rot fungus Cerrena unicolor C-139 produced 450 000 U l(-1) of laccase when cultivated in submerged (50 ml) fermentation of wheat bran. Laccase (benzenediol: oxygen oxidoreductase, EC 1.10.3.2.), from C. unicolor C-139 was immobilized covalently on control porosity carrier silica beads. The activity of the immobilized laccase was approximately 15.8 units per gram of silica beads. The pH optimum was between 2.5 and 3.0 for free and immobilized laccase. The immobilization of enzyme appeared to be the main factor for retention of laccase activity at high temperature of 80 °C. The apparent K(m) value (100 μmol) of immobilized laccase from C. unicolor C-139 was 6.7 times higher than free laccase (15 μmol) using 2,2-azino-bis-[3-ethylthiazoline-6-sulfonate] (ABTS) as the substrate. Immobilized laccase was able to eliminate 80 % of Bisphenol A, 40 % of Nonylphenol, and 60 % of Triclosan from solutions containing 50 μmol of each micropollutant separately. The experiments were run three times consecutively with the same immobilized laccase without loss of enzyme activity.
白腐菌 Cerrena unicolor C-139 在麦麸的液体深层发酵（50ml）中培养时，可产生 450000U l(-1)的漆酶。漆酶（邻苯二酚：氧氧化还原酶，EC 1.10.3.2.）来源于 C. unicolor C-139，被共价固定在控制多孔载体硅胶珠上。固定化漆酶的活性约为每克硅胶珠 15.8 个单位。游离和固定化漆酶的最适 pH 值在 2.5 和 3.0 之间。在 80°C 的高温下，酶的固定化似乎是保持漆酶活性的主要因素。固定化漆酶的表观 K(m)值（100μmol）是游离漆酶（15μmol）的 6.7 倍，以 2,2-联氮双[3-乙基苯并噻唑啉-6-磺酸]（ABTS）为底物。固定化漆酶能够从单独含有 50μmol 每种微污染物的溶液中去除 80%的双酚 A、40%的壬基酚和 60%的三氯生。在相同的固定化漆酶上，连续进行了三次实验，没有酶活性损失。
