Zhejiang-California International Nanosystems Institute, Zhejiang University, Hangzhou, Zhejiang 310029, China.
J Colloid Interface Sci. 2012 Nov 1;385(1):8-14. doi: 10.1016/j.jcis.2012.06.083. Epub 2012 Jul 16.
Monodispersed quantum dots (QDs)-encoded polymer microbeads were generated using a simple capillary fluidic device (CFD). The polymer and QDs solution was emulsified into monodispersed microdroplets by the CFD and obtained droplets were solidified via solvent evaporation. Polymer microbeads can be fabricated in a range of different sizes through changing the flow rates of the two immiscible phases, and have a highly narrow size distribution and uniform shape. QDs-encoding capacity of the microbeads was investigated through adjusting the concentrations and ratios of QDs in the polymer solution. Mono-color encoded microbeads with five intensities and a dual-color QDs-encoded 5×5 microbeads array were obtained, and the spectral profiles of the microbeads were examined by a fluorescent microscope coupled with a spectral imaging system. QDs-tagged microbeads prepared with this method were more stable than the porous beads swollen with QDs in the buffer with various pH and crosslinking chemicals. Finally, the application of such microbeads for biomolecule detection was demonstrated by conjugation of rabbit IgG molecules on the surface of the microbeads via carboxyl groups, which were then detected by fluorophores-labeled goat-anti-rabbit IgG antibodies.
使用简单的毛细管流控装置（CFD）生成了单分散量子点（QD）编码聚合物微球。聚合物和 QD 溶液通过 CFD 乳化成单分散微滴，然后通过溶剂蒸发使获得的液滴固化。通过改变两种不混溶相的流速，可以制备一系列不同尺寸的聚合物微球，并且具有高度狭窄的尺寸分布和均匀的形状。通过调整聚合物溶液中 QD 的浓度和比例来研究微球的编码能力。获得了具有五种强度的单彩色编码微球和双彩色 QD 编码的 5×5 微球阵列，并通过荧光显微镜结合光谱成像系统检查了微球的光谱分布。与在具有各种 pH 值和交联化学物质的缓冲液中溶胀的多孔珠相比，用该方法制备的 QD 标记的微球更稳定。最后，通过羧基将兔 IgG 分子偶联到微球表面，然后通过荧光标记的山羊抗兔 IgG 抗体进行检测，证明了这种微球在生物分子检测中的应用。
