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短发夹 RNA 靶向柯萨奇病毒 B3 的 2B 基因，在体外和体内均显示出潜在的抗病毒作用。

Short hairpin RNA targeting 2B gene of coxsackievirus B3 exhibits potential antiviral effects both in vitro and in vivo.

机构信息

Molecular Immunology Laboratory, Capital Institute of Pediatrics, YaBao Road 2, Beijing, 100020, China.

出版信息

BMC Infect Dis. 2012 Aug 6;12:177. doi: 10.1186/1471-2334-12-177.

DOI:10.1186/1471-2334-12-177
PMID:22863145
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3482581/
Abstract

BACKGROUND

Coxsackievirus B3 is an important infectious agent of viral myocarditis, pancreatitis and aseptic meningitis, but there are no specific antiviral therapeutic reagents in clinical use. RNA interference-based technology has been developed to prevent the viral infection.

METHODS

To evaluate the impact of RNA interference on viral replication, cytopathogenicity and animal survival, short hairpin RNAs targeting the viral 2B region (shRNA-2B) expressed by a recombinant vector (pGCL-2B) or a recombinant lentivirus (Lenti-2B) were tansfected in HeLa cells or transduced in mice infected with CVB3.

RESULTS

ShRNA-2B exhibited a significant effect on inhibition of viral production in HeLa cells. Furthermore, shRNA-2B improved mouse survival rate, reduced the viral tissues titers and attenuated tissue damage compared with those of the shRNA-NC treated control group. Lenti-2B displayed more effective role in inhibition of viral replication than pGCL-2B in vivo.

CONCLUSIONS

Coxsackievirus B3 2B is an effective target of gene silencing against coxsackievirus B3 infection, suggesting that shRNA-2B is a potential agent for further development into a treatment for enterviral diseases.

摘要

背景

柯萨奇病毒 B3 是病毒性心肌炎、胰腺炎和无菌性脑膜炎的重要感染因子，但临床应用中没有特异性抗病毒治疗试剂。基于 RNA 干扰的技术已被开发用于预防病毒感染。

方法

为了评估 RNA 干扰对病毒复制、细胞病变和动物存活的影响，用重组载体（pGCL-2B）或重组慢病毒（Lenti-2B）转染短发夹 RNA（shRNA-2B）靶向病毒 2B 区（shRNA-2B），转导感染 CVB3 的小鼠。

结果

shRNA-2B 对 HeLa 细胞中病毒产生的抑制有显著作用。此外，与 shRNA-NC 处理的对照组相比，shRNA-2B 提高了小鼠的存活率，降低了病毒组织滴度，减轻了组织损伤。与 pGCL-2B 相比，Lenti-2B 在体内对病毒复制的抑制作用更有效。

结论

柯萨奇病毒 B3 2B 是针对柯萨奇病毒 B3 感染的基因沉默的有效靶点，表明 shRNA-2B 是进一步开发用于治疗肠道病毒病的潜在药物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a2d/3482581/7de9d3955bc5/1471-2334-12-177-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a2d/3482581/79a1e62861b5/1471-2334-12-177-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a2d/3482581/14494595ecf7/1471-2334-12-177-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a2d/3482581/9c19b0911964/1471-2334-12-177-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a2d/3482581/ffb3d327df3b/1471-2334-12-177-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a2d/3482581/b49fc9ff0377/1471-2334-12-177-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a2d/3482581/7de9d3955bc5/1471-2334-12-177-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a2d/3482581/79a1e62861b5/1471-2334-12-177-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a2d/3482581/14494595ecf7/1471-2334-12-177-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a2d/3482581/9c19b0911964/1471-2334-12-177-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a2d/3482581/ffb3d327df3b/1471-2334-12-177-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a2d/3482581/b49fc9ff0377/1471-2334-12-177-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a2d/3482581/7de9d3955bc5/1471-2334-12-177-6.jpg

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