Center for Environmental Research and Technology, Bourns College of Engineering, University of California, 1084 Columbia Avenue, Riverside, CA, 92507, USA.
Biotechnol Biofuels. 2012 Aug 3;5(1):56. doi: 10.1186/1754-6834-5-56.
Because many Miscanthus genotypes can be cultivated with relatively high productivity and carbohydrate content, Miscanthus has great potential as an energy crop that can support large scale biological production of biofuels.
In this study, batch hydrothermal pretreatment at 180°C for 35 min followed by enzymatic hydrolysis was shown to give the highest total sugar yields for Miscanthus x giganteus cv. Illinois planted in Illinois. High throughput pretreatment at 180°C for 35 min and 17.5 min followed by co-hydrolysis in a multi-well batch reactor identified two varieties out of 80 that had significantly higher sugar yields from pretreatment and enzymatic hydrolysis than others. The differences in performance were then related to compositions of the 80 varieties to provide insights into desirable traits for Miscanthus that enhance sugar yields.
High throughput pretreatment and co-hydrolysis (HTPH) rapidly identified promising genotypes from a wide range of Miscanthus genotypes, including hybrids of Miscanthus sacchariflorus/M. sinensis and Miscanthus lutarioriparius, differentiating the more commercially promising species from the rest. The total glucan plus xylan content in Miscanthus appeared to influence both mass and theoretical yields, while lignin and ash contents did not have a predictable influence on performance.
由于许多芒属基因型可以以相对较高的生产力和碳水化合物含量进行种植，芒属具有作为能源作物的巨大潜力，可以支持大规模生物生产生物燃料。
在这项研究中，180°C 下 35 分钟的分批热液预处理，随后进行酶水解，显示出在伊利诺伊州种植的芒属巨芒 cv. Illinois 中总糖产量最高。在 180°C 下 35 分钟和 17.5 分钟的高通量预处理，然后在多孔批量反应器中进行共水解，从预处理和酶水解中鉴定出 80 个品种中的两个品种的糖产量明显高于其他品种。然后将性能差异与 80 个品种的组成相关联，以深入了解提高糖产量的芒属理想特性。
高通量预处理和共水解（HTPH）迅速从广泛的芒属基因型中确定了有前途的基因型，包括芒属 sacchariflorus/M.sinensis 和芒属 lutarioriparius 的杂种，将更具商业前景的物种与其他物种区分开来。芒属的总葡聚糖加木聚糖含量似乎同时影响质量和理论产量，而木质素和灰分含量对性能没有可预测的影响。
