Li Zhao-xu, Sun Ling-ling, Cheng Rui-lin, Sun Zheng-wang, Ye Zhao-ming
Department of Orthopaedics, Guilin Medical University, Guilin, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2012 Aug;28(8):822-4.
To investigate the amplification and cytotoxicity of γδ T cells in peripheral blood mononuclear cells (PBMCs) of healthy donors and osteosarcoma patients stimulated by zoledronate (Zol) and IL-2.
PBMCs from healthy donors and osteosarcoma patients were stimulated with IL-2 and Zol+IL-2, respectively. After 14-day culture, the purity of γδ T cells was assessed by flow cytometry. The cytotoxicity of γδ T cells against target cells was analyzed using a standard lactate dehydrogenase release assay with γδ T lymphocyte-sensitive Daudi cells, γδ T lymphocyte-resistant Raji cells and human osteoblast cell line, hFOB, as the target cells.
After 2-week culture ex vivo of PBMCs from healthy donors and osteosarcoma patients, compared with stimulation of IL-2, Zol+IL-2 significantly promoted the amplification of γδ T cells. In addition, γδ T cells showed the higher cytotoxicity against Daudi cells, but no cytotoxic effect on normal cells like hFOB.
γδ T cells of high purity and high cytotoxicity can be obtained by the stimulation of Zol combined with IL-2 on PBMCs from healthy donors and osteosarcoma patients.
研究唑来膦酸（Zol）和白细胞介素-2（IL-2）刺激下健康供者及骨肉瘤患者外周血单个核细胞（PBMCs）中γδT细胞的扩增及细胞毒性。
分别用IL-2和Zol+IL-2刺激健康供者和骨肉瘤患者的PBMCs。培养14天后，通过流式细胞术评估γδT细胞的纯度。以γδT淋巴细胞敏感的Daudi细胞、γδT淋巴细胞耐药的Raji细胞和人成骨细胞系hFOB作为靶细胞，采用标准乳酸脱氢酶释放试验分析γδT细胞对靶细胞的细胞毒性。
健康供者和骨肉瘤患者的PBMCs体外培养2周后，与IL-2刺激相比，Zol+IL-2显著促进γδT细胞的扩增。此外，γδT细胞对Daudi细胞显示出较高的细胞毒性，但对hFOB等正常细胞无细胞毒性作用。
通过Zol联合IL-2刺激健康供者和骨肉瘤患者的PBMCs，可获得高纯度、高细胞毒性的γδT细胞。
