666Nireas, International Water Research Centre, University of Cyprus, 75 Kallipoleos St.1678, Nicosia, Cyprus.
Water Sci Technol. 2012;66(7):1574-81. doi: 10.2166/wst.2012.350.
In this work, a sensitive and highly selective method was developed and validated to study the degradation of two antibiotic compounds (ofloxacin (OFX) and trimethoprim (TMP)), spiked in secondary treated domestic effluents, by the solar Fenton process. Three different chromatographic columns were tested on a ultra performance liquid chromatography-tandem mass spectrometric (UPLC-MS/MS) instrument working in the electrospray ionization (ESI) mode with twelve combinations of eluting solvents. Samples were enriched prior to the analysis by solid phase extraction using the hydrophilic-lipophilic balanced (HLB) reversed phase polymeric sorbent. The method was optimized and showed very good performance characteristics and was successfully applied to study the degradation kinetics of the selected antibiotics during the solar Fenton process applied. The degradation was found to follow a pseudo first-order kinetics for both compounds at initial concentration of 100 μg L(-1) with k = 0.0345 min(-1) for OFX and k = 0.0768 min(-1) for TMP, whereas the complete removal was achieved after 120 min of treatment for both compounds.
在这项工作中，开发并验证了一种灵敏且高度选择性的方法，用于研究在二级处理的生活废水中，通过太阳芬顿工艺降解两种抗生素化合物（氧氟沙星（OFX）和甲氧苄啶（TMP））。在电喷雾电离（ESI）模式下工作的超高效液相色谱-串联质谱（UPLC-MS/MS）仪器上测试了三种不同的色谱柱，共进行了十二种洗脱溶剂组合的测试。通过使用亲水-疏水平衡（HLB）反相聚合吸附剂进行固相萃取，在分析前对样品进行了富集。该方法进行了优化，表现出非常好的性能特征，并成功应用于研究选定抗生素在应用的太阳芬顿工艺过程中的降解动力学。结果表明，在初始浓度为 100μg/L 时，两种化合物的降解均遵循伪一级动力学，OFX 的 k 值为 0.0345 min(-1)，TMP 的 k 值为 0.0768 min(-1)，而在 120 分钟的处理后，两种化合物均完全去除。
