Aydin Osman Nuri, Ek Rauf Onur, Temoçin Sadun, Uğur Bakiye, Alaçam Bilge, Şen Selda
Department of Anesthesiology and Reanimation/Algology, Adnan Menderes University Faculty of Medicine, Aydin, Turkey.
Agri. 2012;24(2):49-55. doi: 10.5505/agri.2012.31032.
The aim of the present study was to investigate the possible antinociceptive effects of systemic administration of tramadol and gabapentin either alone or in combination on acute pain models in mice.
After obtaining the approval of Animal Ethics Committee; 96 BALB/c albino male mice were divided into 12 groups: (I) control without injection, (II) control treated with saline, (III)-(IV) mice treated with tramadol 10 mg/kg or 30 mg/kg, (V)-(VIII) mice treated with gabapentin; 30, 100, 200, 300 mg/kg respectively. In order to determine possible interactions between tramadol gabapentin and; mice received four different combinations of tramadol + gabapentin (30+30, 30+100, 30+200 and 30+300 mg/kg) (Groups IX-XII respectively). Mice received 0.1 ml solution for every 10 g of their weight. The drug was injected into peritonea. Thirty minutes after the drug injection, tail-flick and hot-plate tests were conducted.
Ten and 30 mg/kg tramadol produced dose dependent antinociceptive effect in tail-flick and hot plate tests. Gabapentin had no antinociceptive effect in the tail flick test except 300 mg/kg dose, and had dose dependent antinociceptive effect in hot-plate test. In both tests, various combinations of tramadol and gabapentin produced an antinociceptive effect that is greater than that produced by tramadol and gabapentin alone. But, just 30 mg/kg tramadol + 300 mg/kg gabapentin combination caused statistically significant increase in both tests (p<0.05).
When gabapentin and tramadol were used in combination, gabapentin had no additive antinociceptive effect except for 300 mg/kg in tail-flick and hot-plate tests. Tail-flick test showed that tramadol produced better antinociceptive effect than gabapentin.
本研究旨在探讨全身给予曲马多和加巴喷丁单独或联合使用对小鼠急性疼痛模型可能的镇痛作用。
获得动物伦理委员会批准后；将96只BALB/c白化雄性小鼠分为12组：（I）未注射对照组，（II）生理盐水处理对照组，（III）-（IV）用10mg/kg或30mg/kg曲马多处理的小鼠，（V）-（VIII）分别用30、100、200、300mg/kg加巴喷丁处理的小鼠。为了确定曲马多和加巴喷丁之间可能的相互作用；小鼠接受了曲马多+加巴喷丁的四种不同组合（分别为30+30、30+100、30+200和30+300mg/kg）（分别为IX-XII组）。小鼠每10g体重接受0.1ml溶液。药物经腹腔注射。药物注射30分钟后，进行甩尾和热板试验。
在甩尾和热板试验中，10mg/kg和30mg/kg曲马多产生剂量依赖性镇痛作用。加巴喷丁在甩尾试验中除300mg/kg剂量外无镇痛作用，在热板试验中有剂量依赖性镇痛作用。在两项试验中，曲马多和加巴喷丁的各种组合产生的镇痛作用均大于单独使用曲马多和加巴喷丁产生的作用。但是，仅30mg/kg曲马多+300mg/kg加巴喷丁组合在两项试验中均引起统计学上的显著增加（p<0.05）。
在甩尾和热板试验中，加巴喷丁与曲马多联合使用时，除300mg/kg外无相加镇痛作用。甩尾试验表明，曲马多的镇痛效果优于加巴喷丁。
