Institut national de la recherche scientifique, Centre Eau, Terre et, Environnement, Université du Québec, 490 de la Couronne, Québec (QC), G1K 9A9, Canada.
Bioresour Technol. 2012 Oct;121:425-31. doi: 10.1016/j.biortech.2012.06.075. Epub 2012 Jul 3.
Sterilization, alkaline-thermal and acid-thermal treatments were applied to different sludge solids concentrations (17.0; 22.4; 29.8; 37.3; 44.8 g/L, respectively) and the pre-treated sludge was used as raw material for Serratia sp.1 to produce extracellular polymeric substances (EPS). After 72 h of fermentation, total EPS of 2.3 and 3.4 g/L were produced in sterilized and alkaline-thermal treated sludge as compared to that of 1.5 g/L in acid-thermal treated sludge. Lower EPS were produced at relatively higher solids concentrations (37.3; 44.8 g/L). Broth, crude forms of capsular and slime EPS were extracted from fermented broths and used as conditioning agents by combining with 150 mg of Ca(2+)/L of kaolin suspensions. Maximum flocculation activity of 79.1% and increased dewatering by 52.2% was achieved using broth and crude capsular EPS, respectively. The results demonstrated that EPS having high flocculating capability could be produced using wastewater sludge as sole raw material.
灭菌、堿热和酸热处理应用于不同的污泥固体浓度（分别为 17.0、22.4、29.8、37.3、44.8 g/L），预处理后的污泥被用作 Serratia sp.1 生产细胞外聚合物物质（EPS）的原料。经过 72 h 的发酵，在灭菌和堿热处理的污泥中产生了 2.3 和 3.4 g/L 的总 EPS，而在酸热处理的污泥中仅产生了 1.5 g/L 的 EPS。在相对较高的固体浓度（37.3、44.8 g/L）下，产生的 EPS 较少。从发酵液中提取了胞外聚合物物质（EPS）的粗型和粘性 EPS，并用 150 mg/L 的 Ca(2+)结合高岭土悬浮液将其作为调理剂使用。使用发酵液和粗型胞外多糖 EPS 分别实现了 79.1%的最大絮凝活性和 52.2%的脱水性提高。结果表明，可以使用废水污泥作为唯一原料生产具有高絮凝能力的 EPS。
