Center for Tissue Regeneration, Repair, and Restoration, Palo Alto VA, Palo Alto, CA, United States.
Osteoarthritis Cartilage. 2012 Nov;20(11):1309-15. doi: 10.1016/j.joca.2012.07.018. Epub 2012 Aug 3.
To evaluate the hypothesis that a mechanical stimulus (30-min walk) will produce a change in serum concentrations of cartilage oligomeric matrix protein (COMP) that is associated with cartilage thickness changes on magnetic resonance imaging (MRI).
Serum COMP concentrations were measured by enzyme-linked immunosorbent assay in 17 patients (11 females, age: 59.0±9.2 years) with medial compartment knee osteoarthritis (OA) at study entry immediately before, immediately after, 3.5 h, and 5.5 h after a 30-min walking activity. Cartilage thickness changes in the medial femur and medial tibia were determined from MR images taken at study entry and at 5-year follow-up. Relationships between changes in cartilage thickness and COMP levels, with post-activity concentrations expressed as a percentage of pre-activity levels, were assessed by the calculation of Pearson correlation coefficients and by multiple linear regression analysis, with adjustments for age, sex, and body mass index (BMI).
Changes in COMP levels 3.5 h and 5.5 h post-activity were correlated with changes in cartilage thickness in the medial femur and tibia at the 5-year follow-up. The results were strengthened after analyses were adjusted for age, sex, and BMI. Neither baseline pre-activity COMP levels nor changes in COMP levels immediately post-activity were correlated with cartilage thickness changes.
The results of this study support the hypothesis that a change in COMP concentration induced by a mechanical stimulus is associated with cartilage thinning at 5 years. Mechanically-induced changes in mechano-sensitive biomarkers should be further explored in the context of stimulus-response models to improve the ability to assess OA progression.
评估以下假说，即机械刺激（30 分钟步行）将引起软骨寡聚基质蛋白（COMP）血清浓度的变化，这种变化与磁共振成像（MRI）上的软骨厚度变化相关。
17 例内侧间室膝骨关节炎（OA）患者（女性 11 例，年龄：59.0±9.2 岁）在研究开始时，于步行 30 分钟前、步行结束即刻、3.5 小时和 5.5 小时分别测定血清 COMP 浓度，采用酶联免疫吸附试验法检测。在研究开始时和 5 年随访时，从 MRI 图像上确定内侧股骨和胫骨的软骨厚度变化。通过计算 Pearson 相关系数和多元线性回归分析，评估运动后 COMP 水平的变化与软骨厚度变化之间的关系，用运动后浓度相对于运动前浓度的百分比来表示。调整因素包括年龄、性别和体重指数（BMI）。
运动后 3.5 小时和 5.5 小时 COMP 水平的变化与 5 年随访时内侧股骨和胫骨的软骨厚度变化相关。调整年龄、性别和 BMI 后，结果更为显著。基线运动前 COMP 水平或运动后 COMP 水平的变化均与软骨厚度变化无相关性。
本研究结果支持以下假说，即机械刺激引起的 COMP 浓度变化与 5 年后的软骨变薄有关。应进一步探讨机械刺激引起的机械敏感生物标志物的变化，以改善评估 OA 进展的能力。
