Mündermann Annegret, Dyrby Chris O, Andriacchi Thomas P, King Karen B
Department of Mechanical Engineering, Stanford University, Stanford, CA, USA.
Osteoarthritis Cartilage. 2005 Jan;13(1):34-8. doi: 10.1016/j.joca.2004.09.007.
To test the hypothesis that physiological cyclic loading during a 30-min walking exercise causes an increase in serum cartilage oligomeric matrix protein (COMP) concentration in a healthy population.
Blood samples (5 ml) were drawn from 10 physically active adults immediately before and after, and 0.5h, 1.5h, 3.5h and 5.5h after a 30-min walking exercise on a level outdoor walking track at self-selected normal speed. On a separate day, blood samples were drawn from the same 10 subjects during 6h while they were resting in a chair. Serum COMP concentrations were determined using a commercial enzyme-linked immunosorbent assay (COMP ELISA). An activity monitor was used to record basic time-distance measurements of gait. Serum COMP concentrations within the exercise protocol and within the resting protocol were compared using separate repeated measures analyses of variance (alpha=0.05).
In the exercise protocol, a first increase (9.7%; P=0.003) occurred immediately after the walking exercise. A second increase in serum COMP concentration (7.0%; P=0.024) occurred 5.5h after the walking exercise. In the resting protocol, the concentration at baseline was significantly higher than at all subsequent time points (8.2%; P<0.050). Serum COMP concentration decreased from the 3.5-h to the 5.5-h sample (-4.8%; P=0.012).
Even a moderate walking activity can significantly influence serum COMP concentration. The immediate response points to a diffusion time of COMP fragments from cartilage to the blood of 30 min or less. The response at 5.5h indicates a metabolic delay for COMP in the range of 5h to 6h.
验证以下假设：在30分钟步行锻炼期间的生理周期性负荷会导致健康人群血清软骨寡聚基质蛋白（COMP）浓度升高。
从10名身体活跃的成年人中采集血样（5毫升），分别在30分钟户外水平步道自选正常速度步行锻炼前、锻炼后即刻、锻炼后0.5小时、1.5小时、3.5小时和5.5小时进行采集。在另一天，让这10名受试者坐在椅子上休息6小时期间采集血样。使用商业酶联免疫吸附测定法（COMP ELISA）测定血清COMP浓度。使用活动监测器记录步态的基本时间-距离测量值。使用单独的重复测量方差分析（α=0.05）比较锻炼方案和休息方案中的血清COMP浓度。
在锻炼方案中，步行锻炼后即刻出现首次升高（9.7%；P=0.003）。步行锻炼后5.5小时血清COMP浓度出现第二次升高（7.0%；P=0.024）。在休息方案中，基线浓度显著高于所有后续时间点（8.2%；P<0.050）。血清COMP浓度从3.5小时样本至5.5小时样本下降（-4.8%；P=0.012）。
即使是适度的步行活动也会显著影响血清COMP浓度。即刻反应表明COMP片段从软骨扩散到血液的时间为30分钟或更短。5.5小时的反应表明COMP的代谢延迟在5小时至6小时范围内。
