Natural Resources Canada, Canadian Forest Service, Pacific Forestry Centre, 506 West Burnside Road, Victoria, BC, V8Z 1M5, Canada.
Planta. 2012 Dec;236(6):1725-46. doi: 10.1007/s00425-012-1715-x. Epub 2012 Aug 7.
The proteomic profiles of primary needles from Cr2-resistant and cr2-susceptible Pinus monticola seedlings were analysed post Cronartium ribicola inoculation by 2-DE. One hundred-and-five protein spots exhibiting significant differential expression were identified using LC-MS/MS. Functional classification showed that the most numerous proteins are involved in defence signalling, oxidative burst, metabolic pathways, and other physiological processes. Our results revealed that differential expression of proteins in response to C. ribicola inoculation was genotype- and infection-stage dependent. Responsive proteins in resistant seedlings with incompatible white pine blister rust (WPBR) interaction included such well-characterized proteins as heat shock proteins (HSPs), reactive oxygen species (ROS) scavenging enzymes, and intermediate factors functioning in the signal transduction pathways triggered by well-known plant R genes, as well as new candidates in plant defence like sugar epimerase, GTP-binding proteins, and chloroplastic ribonucleoproteins. Fewer proteins were regulated in susceptible seedlings; most of them were in common with resistant seedlings and related to photosynthesis among others. Quantitative RT-PCR analysis confirmed HSP- and ROS-related genes played an important role in host defence in response to C. ribicola infection. To the best of our knowledge, this is the first comparative proteomics study on WPBR interactions at the early stages of host defence, which provides a reference proteomic profile for other five-needle pines as well as resistance candidates for further understanding of host resistance in the WPBR pathosystem.
采用 2-DE 技术分析了抗 Cr2 和易感染 Cr2 的油松幼苗原生针叶在接种 Cronartium ribicola 后的蛋白质组谱。通过 LC-MS/MS 鉴定出 105 个表现出显著差异表达的蛋白点。功能分类表明，数量最多的蛋白质参与防御信号、氧化爆发、代谢途径和其他生理过程。我们的结果表明，对 C. ribicola 接种的蛋白质差异表达与基因型和感染阶段有关。在与白松疱锈病（WPBR）互作的抗性幼苗中，响应蛋白包括热休克蛋白（HSPs）、活性氧（ROS）清除酶和信号转导途径中的中间因子等，这些蛋白在已知的植物 R 基因触发的信号转导途径中发挥作用，以及植物防御中的新候选物，如糖差向异构酶、GTP 结合蛋白和质体核糖核蛋白。易感幼苗中调节的蛋白质较少；其中大多数与抗性幼苗相同，与光合作用等有关。定量 RT-PCR 分析证实，HSP 和 ROS 相关基因在宿主防御中对 C. ribicola 感染的反应中发挥了重要作用。据我们所知，这是宿主防御早期 WPBR 相互作用的首次比较蛋白质组学研究，为其他五针松提供了参考蛋白质组谱，并为进一步了解 WPBR 病理系统中的宿主抗性提供了抗性候选物。
