Jin T, Zeng L, Lin Y-Y, Lu Y-Y, Liang G-W
Laboratory of Insect Ecology, College of Natural Resources and Enviornment, South China Agricultural University, Guangzhou, China.
Genet Mol Res. 2012 Aug 16;11(3):2608-19. doi: 10.4238/2012.July.10.12.
Functional proteins in larvae of Bactrocera dorsalis, a major fruit pest, play a central role in their resistance to organophosphorus insecticides. Changes in proteins in B. dorsalis larvae after trichlorphon treatment may have a role in the resistance response to trichlorphon. We analyzed 14 protein spots of crude proteins from B. dorsalis larvae post-treatment with trichlorphon in two-dimensional gel electrophoresis through mass spectrometry and protein sequencing. We found functional proteins that are responsible for signal transduction (pkaap and dual specificity tyrosine-phosphorylation-regulated kinase), immunity (hemolectin), synthesis and decomposition (twinstar, cathepsin B, RE66325p), oxidative stress metabolism (glutathione S transferase and CG7320), energy metabolism (Act57B), and cytoskeleton formation (actin). These proteins appear to be involved in the resistance response to trichlorphon.
桔小实蝇是一种主要的水果害虫，其幼虫体内的功能蛋白在对有机磷杀虫剂的抗性中起着核心作用。经敌百虫处理后，桔小实蝇幼虫体内蛋白质的变化可能在对敌百虫的抗性反应中发挥作用。我们通过质谱分析和蛋白质测序，在二维凝胶电泳中分析了经敌百虫处理后的桔小实蝇幼虫粗蛋白中的14个蛋白点。我们发现了负责信号转导（pkaap和双特异性酪氨酸磷酸化调节激酶）、免疫（血凝集素）、合成与分解（双星蛋白、组织蛋白酶B、RE66325p）、氧化应激代谢（谷胱甘肽S-转移酶和CG7320）、能量代谢（Act57B）以及细胞骨架形成（肌动蛋白）的功能蛋白。这些蛋白似乎参与了对敌百虫的抗性反应。
