Yano Akira, Shigematsu Yoshinori, Kitano Hiroyuki, Hanayama Aki, Ozawa Akira, Tacho Takatoshi, Fujii Motohiro
Department of Urology, Matsuyama Red Cross Hospital, Ehime 790-8524, Japan.
Oncol Lett. 2010 Nov;1(6):1049-1053. doi: 10.3892/ol.2010.165. Epub 2010 Sep 1.
Thymidylate synthase (TS), dihydropyrimidine dehydrogenase (DPD), thymidine phosphorylase (TP) and orotate phosphoribosyl transferase (OPRT) are enzymes involved in nucleic acid metabolism. It has been reported (based on observations of various tumor types) that the extent of the mRNA expression of these enzymes within tumor tissues may be used as a factor to define tumor prognosis. It has also been reported that the mRNA expression patterns differ in each type of tumor. However, few reports are available on the distribution of mRNA expression in prostate cancers. This study was conducted on tissue specimens obtained from 172 patients who were diagnosed with prostate cancer and had undergone total prostatectomies. The mRNA expression of TS, DPD, OPRT and TP was quantitatively analyzed using the Danenberg tumor profile (DTP) method. The results were used to examine the correlations between the distributions of the mRNAs and clinicopathological factors, as well as the significance of their expression as a prognostic factor. Patients with poorly differentiated cancers in their tissues showed a significant increase in the mRNA expression of TS and OPRT. The increases in the TP mRNA content were proportional to an increase in the Gleason scores. The prognosis was significantly poorer in those cases with a high expression of TS or OPRT mRNA and a low expression of DPD mRNA. In conclusion, the expression levels of mRNAs for TS, DPD and OPRT among the enzymes related to nucleic acid metabolism are useful as prognostic factors in patients with prostate cancers.
胸苷酸合成酶（TS）、二氢嘧啶脱氢酶（DPD）、胸苷磷酸化酶（TP）和乳清酸磷酸核糖转移酶（OPRT）是参与核酸代谢的酶。据报道（基于对各种肿瘤类型的观察），这些酶在肿瘤组织内的mRNA表达程度可作为定义肿瘤预后的一个因素。也有报道称，每种肿瘤类型的mRNA表达模式有所不同。然而，关于前列腺癌中mRNA表达分布的报道较少。本研究对172例被诊断为前列腺癌并接受了前列腺全切术的患者的组织标本进行了研究。使用达嫩伯格肿瘤谱（DTP）方法对TS、DPD、OPRT和TP的mRNA表达进行了定量分析。结果用于检验mRNA分布与临床病理因素之间的相关性，以及它们作为预后因素表达的意义。组织中癌症分化差的患者TS和OPRT的mRNA表达显著增加。TP mRNA含量的增加与Gleason评分的增加成正比。TS或OPRT mRNA高表达且DPD mRNA低表达的病例预后明显较差。总之，在与核酸代谢相关的酶中，TS、DPD和OPRT的mRNA表达水平可作为前列腺癌患者的预后因素。
