College of Pharmacy, Sahmyook University, Seoul 139-742, Korea.
Arch Pharm Res. 2012 Jun;35(6):1065-71. doi: 10.1007/s12272-012-0614-9. Epub 2012 Jun 30.
The objective of this study was to evaluate the antibacterial and anti-inflammatory effect of Bifidobacterium spp. In the first part of the study, the antibacterial activities of live and sonicated cells, from a total of 23 Bifidobacterium species, on the growth of 5 different strain of Staphylococcus aureus. Six strains, of sonicated Bifidobacterium, exhibited antibacterial activity against staphylococci samples, and seven Bifidobacterium strains exhibited antibacterial activity on the growth of S. aureus S.P.-N2. In the second part of the study, we tested the antimicrobial activity, of Bifidobacterium against Propionibacterium acne KCTC3320, using the co-culture method. The loss of P. acnes viability, caused by B. adolescentis SPM0308 and B. longum SPM1207, was 84% and 75%, respectively (*p < 0.05). In the third part of the study, the anti-inflammatory activity of B. adolescentis SPM0308 and B. longum SPM1207 were assessed; nitric oxide (NO), and tumor necrosis factor-α (TNF-α), production were tested using the murine macrophage RAW 264.7 cell line. Treatment of RAW 264.7 cells, with Bifidobacterium, decreased production of NO and TNF-α rather than LPS (100 ng/mL) treatment. The results suggest that B. adolescentis SPM0308 could be used as an effective control for P. acnes KCTC3320, and S. aureus, and reduce the risk of acne vulgaris development. We suggest that B. adolescentis SPM0308 may be a useful probiotic microorganism, for prevention of acne vulgaris, without adverse effects.
本研究的目的是评估双歧杆菌属的抗菌和抗炎作用。在研究的第一部分，对来自 23 种双歧杆菌属的活菌和超声细胞，对 5 株不同金黄色葡萄球菌株的生长的抗菌活性进行了评估。6 株超声双歧杆菌对葡萄球菌样品具有抗菌活性，7 株双歧杆菌对金黄色葡萄球菌 S.P.-N2 的生长具有抗菌活性。在研究的第二部分，我们使用共培养法测试了双歧杆菌对痤疮丙酸杆菌 KCTC3320 的抗菌活性。双歧杆菌短双歧杆菌 SPM0308 和长双歧杆菌 SPM1207 导致痤疮丙酸杆菌活力丧失分别为 84%和 75%（*p < 0.05）。在研究的第三部分，评估了双歧杆菌短双歧杆菌 SPM0308 和长双歧杆菌 SPM1207 的抗炎活性；使用鼠巨噬细胞 RAW 264.7 细胞系测试了一氧化氮（NO）和肿瘤坏死因子-α（TNF-α）的产生。用双歧杆菌处理 RAW 264.7 细胞，降低了 NO 和 TNF-α的产生，而不是 LPS（100ng/ml）处理。结果表明，双歧杆菌短双歧杆菌 SPM0308 可有效控制痤疮丙酸杆菌 KCTC3320 和金黄色葡萄球菌，降低寻常痤疮发展的风险。我们建议双歧杆菌短双歧杆菌 SPM0308 可能是一种有效的预防寻常痤疮的益生菌微生物，没有不良反应。
