Alontaga Aileen Y, Bobkova Ekaterina, Chen Yuan
Department of Molecular Medicine, Beckman Research Institute of the City of Hope, Duarte, California, USA.
Curr Protoc Mol Biol. 2012 Jul;Chapter 10:Unit10.29. doi: 10.1002/0471142727.mb1029s99.
SUMOylation, the covalent attachment of Small Ubiquitin-like MOdifier (SUMO) polypeptides to other proteins, is among the most important post-translational modifications that regulate the functional properties of a large number of proteins. SUMOylation is broadly involved in cellular processes such as gene transcription, hormone response, signal transduction, DNA repair, and nuclear transport. SUMO modification has also been implicated in the pathogenesis of human diseases, such as cancer, neurodegenerative disorders, and viral infection. Attachment of a SUMO protein to another protein is carried out in multiple steps catalyzed by three enzymes. This unit describes and discusses the in vitro biochemical methods used for investigating each step of the SUMOylation process. In addition, a high-throughput screening protocol is included for the identification of inhibitors of SUMOylation.
SUMO化是指小泛素样修饰物（SUMO）多肽与其他蛋白质的共价连接，是调节大量蛋白质功能特性的最重要的翻译后修饰之一。SUMO化广泛参与细胞过程，如基因转录、激素反应、信号转导、DNA修复和核运输。SUMO修饰也与人类疾病的发病机制有关，如癌症、神经退行性疾病和病毒感染。SUMO蛋白与另一种蛋白质的连接是由三种酶催化的多个步骤完成的。本单元描述并讨论了用于研究SUMO化过程各个步骤的体外生化方法。此外，还包括一个用于鉴定SUMO化抑制剂的高通量筛选方案。
