海洋反硝化菌完美海洋假单胞菌细胞质膜组分将亚硝酸盐还原为一氧化二氮。
Reduction of nitrite to nitrous oxide by a cytoplasmic membrane fraction from the marine denitrifier Pseudomonas perfectomarinus.
作者信息
Zumft W G, Vega J M
出版信息
Biochim Biophys Acta. 1979 Dec 6;548(3):484-99. doi: 10.1016/0005-2728(79)90060-4.
A cytoplasmic membrane fraction from the marine denitrifier Pseudomonas perfectomarinus reduced nitrite to nitrous oxide in a stoichiometric reaction without nitric oxide as free intermediate. The membrane system had a specific requirement for FMN with NAD(P)H as electron donors. Other electron donors were ascorbate-reduced cytochrome c-551 or phenazine methosulfate. The membrane fraction contained tightly bound cytochrome cd which represented only a small portion of the total cytochrome cd of the cell. As further terminal oxidase cytochrome o was identified. The membrane fraction produced also nitrous oxide from nitric oxide, however, at a substantially lower rate than from nitrite when using ascorbate-reduced phenazine methosulfate as electron donor.
来自海洋反硝化细菌完美海洋假单胞菌的细胞质膜组分，在无一氧化氮作为游离中间体的化学计量反应中，将亚硝酸盐还原为一氧化二氮。该膜系统对以NAD(P)H作为电子供体的黄素单核苷酸有特定需求。其他电子供体是抗坏血酸还原的细胞色素c-551或吩嗪硫酸甲酯。该膜组分含有紧密结合的细胞色素cd，其仅占细胞总细胞色素cd的一小部分。作为进一步的末端氧化酶，细胞色素o被鉴定出来。当使用抗坏血酸还原的吩嗪硫酸甲酯作为电子供体时，该膜组分也能将一氧化氮转化为一氧化二氮，然而，其反应速率比从亚硝酸盐转化时要低得多。
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