Zumft W G, Vega J M
Biochim Biophys Acta. 1979 Dec 6;548(3):484-99. doi: 10.1016/0005-2728(79)90060-4.
A cytoplasmic membrane fraction from the marine denitrifier Pseudomonas perfectomarinus reduced nitrite to nitrous oxide in a stoichiometric reaction without nitric oxide as free intermediate. The membrane system had a specific requirement for FMN with NAD(P)H as electron donors. Other electron donors were ascorbate-reduced cytochrome c-551 or phenazine methosulfate. The membrane fraction contained tightly bound cytochrome cd which represented only a small portion of the total cytochrome cd of the cell. As further terminal oxidase cytochrome o was identified. The membrane fraction produced also nitrous oxide from nitric oxide, however, at a substantially lower rate than from nitrite when using ascorbate-reduced phenazine methosulfate as electron donor.
来自海洋反硝化细菌完美海洋假单胞菌的细胞质膜组分，在无一氧化氮作为游离中间体的化学计量反应中，将亚硝酸盐还原为一氧化二氮。该膜系统对以NAD(P)H作为电子供体的黄素单核苷酸有特定需求。其他电子供体是抗坏血酸还原的细胞色素c-551或吩嗪硫酸甲酯。该膜组分含有紧密结合的细胞色素cd，其仅占细胞总细胞色素cd的一小部分。作为进一步的末端氧化酶，细胞色素o被鉴定出来。当使用抗坏血酸还原的吩嗪硫酸甲酯作为电子供体时，该膜组分也能将一氧化氮转化为一氧化二氮，然而，其反应速率比从亚硝酸盐转化时要低得多。
