School of Life Science, Fuyang Teachers College, Anhui Province, Fuyang, China; Key Laboratory of Embryo Development and Reproductive Regulation, Anhui Province, Fuyang, China.
Gene. 2012 Oct 10;507(2):119-24. doi: 10.1016/j.gene.2012.07.030. Epub 2012 Jul 31.
Ifrg15 is a newly identified interferon alpha responsive gene and is implicated in a wide variety of physiological roles in mammals. In the present study, multiple alignments of the deduced amino acids of 10 eutherian mammalian IFRG15/Ifrg15s isolated from open genomic database revealed that they were highly conserved. Real-time PCR showed that mouse Ifrg15 mRNA was expressed in MII stage oocytes and preimplantation embryos, and its highest value peaked at the stage of mouse blastocysts. To understand the effect of three development-related genes on the promoter activity of mouse Ifrg15, promoter analysis using luciferase assays in COS-7 cells were performed. The results showed that the transcription of mouse Ifrg15 was suppressed by Oct4 and Nanog when transfected with the longest Ifrg15 promoter reporter gene. After the relatively shorter promoters were co-transfected with Oct4, c-Myc and Nanog, the relative luciferase activities of Ifrg15 were gradually increased. These in vitro results data and expression profiles of Ifrg15 as revealed by real-time PCR partly indicated that Ifrg15 transcription might be either potentially regulated or dependent on the post-transcriptional effects of IFN-α mediated by the three genes indirectly. Our data suggested that the mouse Ifrg15 might interact with these key development-related genes and play significant roles on the mouse preimplantation embryos development, especially for the development of mouse blastocysts.
IFRG15 是一个新鉴定的干扰素α反应基因，涉及哺乳动物的多种生理功能。本研究通过对从开放基因组数据库中分离的 10 种真兽类 IFRG15/Ifrg15 的推导氨基酸进行多重比对，发现它们高度保守。实时 PCR 显示，小鼠 Ifrg15 mRNA 在 MII 期卵母细胞和植入前胚胎中表达，其最高值在小鼠囊胚阶段达到峰值。为了了解三个发育相关基因对小鼠 Ifrg15 启动子活性的影响，在 COS-7 细胞中进行了荧光素酶检测的启动子分析。结果表明，当转染最长的 Ifrg15 启动子报告基因时，Oct4 和 Nanog 抑制了小鼠 Ifrg15 的转录。当相对较短的启动子与 Oct4、c-Myc 和 Nanog 共转染时，Ifrg15 的相对荧光素酶活性逐渐增加。这些体外结果数据和实时 PCR 揭示的 Ifrg15 表达谱部分表明，Ifrg15 的转录可能受到 IFN-α介导的三个基因的转录后效应的潜在调节或依赖。我们的数据表明，小鼠 Ifrg15 可能与这些关键的发育相关基因相互作用，在小鼠植入前胚胎发育中发挥重要作用，特别是在小鼠囊胚的发育中。
