Laboratoire PROTEE-EBMA (EA 3819), Université du SUD TOULON VAR, Avenue de l'Université, BP 20132, F-83957 La Garde, Cedex, France.
Mar Pollut Bull. 2012 Oct;64(10):1989-96. doi: 10.1016/j.marpolbul.2012.07.028. Epub 2012 Aug 4.
The regulation of alkaline phosphatase activity by dissolved inorganic (DIP) and organic phosphorus (DOP) and the contribution of DOP as phosphorus source were studied monthly in Toulon Bay (NW Mediterranean, France) in 2005-2006. The concentrations of DIP and DOP varied respectively from 0 to 0.185μM and from 0 to 0.329μM. The bulk activities (Vm, Km, Vm/Km) were measured using MUFP as substrate. Its high affinity component (Km: 0.05-1.00μM) was negatively correlated with the sum of the concentrations of DIP and DOP but not with these compounds taken independently. A negative correlation with DIP was found when the concentrations of DOP were lower than 0.08μM. A negative correlation with DOP was shown when the concentrations of DIP were lower than 0.05μM. This high affinity component can be considered as a valuable indicator for the potential utilization of the compounds which contribute to the intracellular phosphorus pool.
2005 年至 2006 年，在法国西北地中海的土伦湾每月研究了溶解无机磷（DIP）和有机磷（DOP）对碱性磷酸酶活性的调节作用，以及 DOP 作为磷源的贡献。DIP 和 DOP 的浓度分别在 0 到 0.185μM 和 0 到 0.329μM 之间变化。使用 MUFP 作为底物测量了总酶活性（Vm、Km、Vm/Km）。其高亲和力组分（Km：0.05-1.00μM）与 DIP 和 DOP 的浓度总和呈负相关，但与这些化合物的独立浓度无关。当 DOP 的浓度低于 0.08μM 时，与 DIP 呈负相关。当 DIP 的浓度低于 0.05μM 时，与 DOP 呈负相关。这个高亲和力组分可以被认为是对有助于细胞内磷池的化合物的潜在利用的有价值的指标。
