Department of Cardiology, The Fifth Affiliated Hospital, Guangzhou Medical University, Guangzhou 510700, China.
Rheumatol Int. 2013 Apr;33(4):1013-20. doi: 10.1007/s00296-012-2455-8. Epub 2012 Aug 8.
We designed and recombined the polypeptide based on the M protein of group A streptococci (GAS)--the causative pathogen of rheumatic fever and rheumatic heart disease, which would be a divalent vaccine to prevent and defend the diseases in relation to the different GAS strains. A divalent vaccine comprising three different peptide epitopes of the antiphagocytic M protein of GAS--an aminoterminal specific sequences, respectively, from the M1 and M12 proteins and J14 peptide (ASREAKKQVEKALE) within the highly conserved C-terminal repeat region of the M1 and M12 proteins--was subcutaneously delivered to mice with the adjuvant. Furthermore, the antisera titers of mice inoculated with the divalent vaccine were assayed by ELISA, and then opsonization and percentage killing against two different GAS serotypes were completed. Our data demonstrated that antisera raised against the divalent vaccine containing amino acids and M-protein-conserved C repeat region are able to kill several GAS strains isolated from the Guangzhou population. Therefore, the divalent vaccine can be used to prevent those diseases caused by GAS in an endemic area. We successfully construct the M-protein-based divalent vaccine that can bring out a high-level antisera titer of mice vaccinated with it. So, the vaccine has the potential to be used to prevent diseases caused by GAS in our country.
我们设计并重组了基于 A 组链球菌（GAS）的 M 蛋白的多肽，GAS 是风湿热和风湿性心脏病的致病病原体，这将是一种二价疫苗，可以预防和抵御与不同 GAS 菌株相关的疾病。一种二价疫苗包含 GAS 抗吞噬 M 蛋白的三个不同肽表位 - 分别来自 M1 和 M12 蛋白的氨基末端特异性序列，以及位于 M1 和 M12 蛋白高度保守的 C 末端重复区的 J14 肽（ASREAKKQVEKALE） - 与佐剂一起皮下递送至小鼠。此外，通过 ELISA 测定接种二价疫苗的小鼠的血清效价，然后完成对两种不同 GAS 血清型的调理作用和杀伤百分比。我们的数据表明，针对包含氨基酸和 M 蛋白保守 C 重复区的二价疫苗产生的抗血清能够杀死从广州人群中分离出的几种 GAS 菌株。因此，该二价疫苗可用于预防流行地区由 GAS 引起的疾病。我们成功构建了基于 M 蛋白的二价疫苗，该疫苗能够引起接种小鼠的高水平血清效价。因此，该疫苗有可能在我国用于预防 GAS 引起的疾病。
