端粒酶核糖核蛋白的生物发生。
Biogenesis of telomerase ribonucleoproteins.
机构信息
Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720-3200, USA.
出版信息
RNA. 2012 Oct;18(10):1747-59. doi: 10.1261/rna.034629.112. Epub 2012 Aug 8.
Abstract
Telomerase adds simple-sequence repeats to the ends of linear chromosomes to counteract the loss of end sequence inherent in conventional DNA replication. Catalytic activity for repeat synthesis results from the cooperation of the telomerase reverse transcriptase protein (TERT) and the template-containing telomerase RNA (TER). TERs vary widely in sequence and structure but share a set of motifs required for TERT binding and catalytic activity. Species-specific TER motifs play essential roles in RNP biogenesis, stability, trafficking, and regulation. Remarkably, the biogenesis pathways that generate mature TER differ across eukaryotes. Furthermore, the cellular processes that direct the assembly of a biologically functional telomerase holoenzyme and its engagement with telomeres are evolutionarily varied and regulated. This review highlights the diversity of strategies for telomerase RNP biogenesis, RNP assembly, and telomere recruitment among ciliates, yeasts, and vertebrates and suggests common themes in these pathways and their regulation.
摘要
端粒酶将简单重复序列添加到线性染色体的末端，以抵消常规 DNA 复制中固有的末端序列丢失。重复合成的催化活性源自端粒酶逆转录酶蛋白 (TERT) 和包含模板的端粒酶 RNA (TER) 的合作。TER 在序列和结构上差异很大，但共享一组 TERT 结合和催化活性所必需的基序。物种特异性 TER 基序在 RNP 生物发生、稳定性、运输和调节中发挥重要作用。值得注意的是，生成成熟 TER 的生物发生途径在真核生物中有所不同。此外，指导生物功能端粒酶全酶组装及其与端粒结合的细胞过程在进化上是多样化和调节的。本综述强调了纤毛虫、酵母和脊椎动物中端粒酶 RNP 生物发生、RNP 组装和端粒募集的策略的多样性，并提出了这些途径及其调节中的共同主题。
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本文引用的文献
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Structural basis for telomerase RNA recognition and RNP assembly by the holoenzyme La family protein p65.端粒酶 RNA 识别和 RNP 组装的结构基础由全酶 La 家族蛋白 p65 完成。
Mol Cell. 2012 Jul 13;47(1):16-26. doi: 10.1016/j.molcel.2012.05.018. Epub 2012 Jun 14.
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Telomerase recruitment requires both TCAB1 and Cajal bodies independently.端粒酶的募集需要 TCAB1 和 Cajal 体的独立作用。
Mol Cell Biol. 2012 Jul;32(13):2384-95. doi: 10.1128/MCB.00379-12. Epub 2012 Apr 30.
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An enhanced H/ACA RNP assembly mechanism for human telomerase RNA.人端粒酶 RNA 的增强 H/ACA RNP 组装机制。
Mol Cell Biol. 2012 Jul;32(13):2428-39. doi: 10.1128/MCB.00286-12. Epub 2012 Apr 23.
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Telomerase RNA biogenesis involves sequential binding by Sm and Lsm complexes.端粒酶 RNA 的生物发生涉及 Sm 和 Lsm 复合物的顺序结合。
Nature. 2012 Mar 25;484(7393):260-4. doi: 10.1038/nature10924.
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Budding yeast telomerase RNA transcription termination is dictated by the Nrd1/Nab3 non-coding RNA termination pathway.芽殖酵母端粒酶 RNA 转录终止由 Nrd1/Nab3 非编码 RNA 终止途径决定。
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Tel1(ATM) and Rad3(ATR) phosphorylate the telomere protein Ccq1 to recruit telomerase and elongate telomeres in fission yeast.Tel1（ATM）和 Rad3（ATR）磷酸化端粒蛋白 Ccq1，以招募端粒酶并在裂殖酵母中延长端粒。
Genes Dev. 2012 Feb 1;26(3):241-6. doi: 10.1101/gad.177873.111.
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