Toutain H, Courjault F, Vauclin-Jacques N, Morin J P
INSERM U-295, UER Médecine-Pharmacie de Rouen, Saint Etienne du Rouvray, France.
Ren Fail. 1990;12(3):177-82. doi: 10.3109/08860229009065561.
Freshly isolated rabbit proximal tubules (PT), confluent primary rabbit proximal tubule cultures (PTC) and LLC-PK1 cells were characterised. Brushborder enzyme activities were lower in PTC than in LLC-PK1: ratios were 0.026 for alkaline phosphatase (AP), 0.458 for alanine aminopeptidase (AAP) and 0.514 for gamma-glutamyl transpeptidase (GGT). PT/PTC ratios were 79.7 for AP, 7.96 for AAP and 3.45 for GGT. Specific activities of hexokinase (HK) and lactate dehydrogenase (LDH) were high in cultured cells as compared to PT: PT/PTC ratios were 0.063 and 0.033, while PTC/LLC-PK1 ratios were 0.406 and 1.19 for HK and LDH respectively. PTC/LLC-PK1 ratios were 2.21 for Na/K ATPase, 2.07 for succinate dehydrogenase, 1.12 for cathepsin B, 0.607 for N-acetyl-beta-D-glucosaminidase and 8.98 for glutathione-S-transferase. Adenylate cyclase response to parathormone (PTH), was similar in PTC and PT, but stimulated/basal ratios were higher in PT than in PTC. LLC-PK1 cells were stimulated by thyrocalcitonin (SCT), arginin-vasopressin (AVP) and PTH; stimulated/basal ratios ranked AVP greater than PTH greater than SCT. Differences between both types of cultures affect the choice of in vitro model for nephrotoxicity studies.
对新鲜分离的兔近端小管（PT）、汇合的原代兔近端小管培养物（PTC）和LLC-PK1细胞进行了特性分析。PTC中的刷状缘酶活性低于LLC-PK1：碱性磷酸酶（AP）的比率为0.026，丙氨酸氨基肽酶（AAP）为0.458，γ-谷氨酰转肽酶（GGT）为0.514。PT/PTC的比率AP为79.7，AAP为7.96，GGT为3.45。与PT相比，培养细胞中的己糖激酶（HK）和乳酸脱氢酶（LDH）的比活性较高：PT/PTC的比率HK为0.063，LDH为0.033，而PTC/LLC-PK1的比率HK为0.406，LDH为1.19。PTC/LLC-PK1的比率钠钾ATP酶为2.21，琥珀酸脱氢酶为2.07，组织蛋白酶B为1.12，N-乙酰-β-D-氨基葡萄糖苷酶为0.607，谷胱甘肽-S-转移酶为8.98。甲状旁腺激素（PTH）对腺苷酸环化酶的反应在PTC和PT中相似，但PT中的刺激/基础比率高于PTC。LLC-PK1细胞受到甲状腺降钙素（SCT）、精氨酸加压素（AVP）和PTH的刺激；刺激/基础比率排序为AVP大于PTH大于SCT。两种培养类型之间的差异影响了肾毒性研究体外模型的选择。
