Narukawa Tomohiro, Hioki Akiharu, Chiba Koichi
National Metrology Institute of Japan, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan.
Anal Sci. 2012;28(8):773-9. doi: 10.2116/analsci.28.773.
An aqueous extraction of inorganic As species, such as arsenite (As(III)) and arsenate (As(V)), was developed for monitoring inorganic As in the edible brown alga Hizikia fusiforme (hijiki). The ultrasonic extraction with water, even without heating, was found to be an acceptable monitoring method for an evaluation of water-soluble inorganic As, since it could extract about 80% of total As. Such an extraction efficiency was almost the same as those of enzyme assisted extraction methods. The developed extraction procedure was applied to 15 hijiki samples that had been collected at different coasts in Japan. All samples contained a substantial proportion of As as arsenosugars; the relative amounts of the different As species extracted were dependent on the sample. The percentages of extractable As species in the hijiki samples were in the range from 70 to 90%, and the sums of the concentrations of As(III) and As(V), which was defined as i-As, were in the range from 36 to 79% of the total As concentration in each sample. The proposed method is appropriate for environmental monitoring for inorganic As speciation in algae.
开发了一种用于提取无机砷物种（如亚砷酸盐（As(III)）和砷酸盐（As(V)））的水相萃取方法，用于监测可食用褐藻羊栖菜中的无机砷。发现即使不加热，用水进行超声萃取也是评估水溶性无机砷的一种可接受的监测方法，因为它可以提取约80%的总砷。这种萃取效率与酶辅助萃取方法的萃取效率几乎相同。所开发的萃取程序应用于在日本不同海岸采集的15个羊栖菜样品。所有样品中大部分砷以砷糖形式存在；提取的不同砷物种的相对含量取决于样品。羊栖菜样品中可萃取砷物种的百分比在70%至90%之间，As(III)和As(V)的浓度总和（定义为i-As）占每个样品总砷浓度的36%至79%。所提出的方法适用于藻类中无机砷形态的环境监测。
