Paul S M, Axelrod J, Saavedra J M, Skolnick P
Brain Res. 1979 Dec 14;178(2-3):499-505. doi: 10.1016/0006-8993(79)90709-1.
Short-term organ cultures of the intact hypothalamus were used to study the effects of various estrogenic compounds on catecholamine release. Estradiol-17 beta (0.1--20 microM) produced a concentration-dependent efflux of norepinephrine and dopamine while its biologically inactive enantiomer, estradiol-17 alpha, was ineffective at concentrations up to 20 microM. Diethylstilbestrol, a potent non-steroidal estrogen, was as effective as estradiol-17 beta in inducing catecholamine efflux. In contrast, weakly or non-estrogenic steroids such as estrone, estriol, and corticosterone were without effect. The time course of the estrogen-induced efflux of hypothalamic catecholamines was similar to that previously reported for the estrogen-induced accumulation of hypothalamic cAMP, providing further evidence for the involvement of catecholamines in this effect. Theses results suggest that estrogen may facilitate the release of catecholamines within the hypothalamus.
利用完整下丘脑的短期器官培养来研究各种雌激素化合物对儿茶酚胺释放的影响。17β-雌二醇（0.1 - 20微摩尔）可引起去甲肾上腺素和多巴胺呈浓度依赖性流出，而其无生物活性的对映体17α-雌二醇在浓度高达20微摩尔时无效。己烯雌酚是一种强效非甾体雌激素，在诱导儿茶酚胺流出方面与17β-雌二醇效果相同。相比之下，弱雌激素或非雌激素类固醇如雌酮、雌三醇和皮质酮则无作用。雌激素诱导的下丘脑儿茶酚胺流出的时间进程与先前报道的雌激素诱导的下丘脑环磷酸腺苷积累的时间进程相似，这为儿茶酚胺参与此效应提供了进一步证据。这些结果表明，雌激素可能促进下丘脑内儿茶酚胺的释放。
