Schoener E P, Hager P J, Felt B T, Schneider D R
Brain Res. 1979 Dec 21;179(1):111-9. doi: 10.1016/0006-8993(79)90495-5.
A push-pull perfusion technique was employed for in vivo study of adenosine 3',5'-monophosphate (cAMP) and guanosine 3',5'-monophosphate (cGMP) in the rat caudate nucleus. Addition of dopamine to the perfusion fluid elicited dose-dependent increases of both cAMP and cGMP perfusate concentrations. In separate experiments, it was found that pretreatment of animals with the dopamine antagonist, pimozide, significantly depressed both nucleotide responses to dopamine perfusion over the dose range studied. Mechanistic interpretations of the observations are considered. The push-pull perfusion technique appears to provide an extremely useful means of examining extracellular cyclic nucleotide levels in a discrete brain region, in vivo, under dynamic conditions.
采用推挽灌注技术对大鼠尾状核中的3',5'-环磷酸腺苷（cAMP）和3',5'-环磷酸鸟苷（cGMP）进行体内研究。向灌注液中添加多巴胺会引起cAMP和cGMP灌注液浓度呈剂量依赖性增加。在单独的实验中发现，用多巴胺拮抗剂匹莫齐特对动物进行预处理，在所研究的剂量范围内，会显著抑制对多巴胺灌注的两种核苷酸反应。对这些观察结果进行了机制解释。推挽灌注技术似乎提供了一种极其有用的方法，可在动态条件下在体内检测离散脑区的细胞外环核苷酸水平。
