Santamaría L, Reoyo A, Regadera J, Paniagua R
Department of Morphology, School of Medicine, Autonomous University of Madrid, Spain.
Acta Anat (Basel). 1990;139(2):126-33. doi: 10.1159/000146988.
Whole-mounted preparations of the tunica albuginea of the rat testis were studied using light microscopy techniques for demonstration of cholinergic nerve fibres (Karnovski-Root method), catecholaminergic nerve fibres (De la Torre's method) and actin filaments (avidin-biotin-peroxidase method). An ultrastructural study of different regions of the albuginea was also performed. Cholinergic fibres were seen to penetrate into the albuginea with the testicular artery to form a broad network in the mediastinum testis, many fibres ending beneath the rete testis epithelium. Catecholaminergic fibres penetrated through the middle part of the cauda epididymis and formed a plexus in the albuginea covering the inferior testicular pole. This plexus gave rise to straight fibres which formed varicosities, some of them appeared related with mast cells. Actin-containing cells were only found beneath the rete testis epithelium. These cells were similar to myofibroblasts. The location of both cholinergic fibres and contractile cells among the rete testis channels suggests that these cells may be involved in the pumping of semen towards the ductuli efferentes and that their contractility may be regulated by cholinergic fibres.
利用光学显微镜技术，对大鼠睾丸白膜整装标本进行研究，以显示胆碱能神经纤维（卡诺夫斯基-鲁特法）、儿茶酚胺能神经纤维（德拉托雷法）和肌动蛋白丝（抗生物素蛋白-生物素-过氧化物酶法）。同时还对白膜不同区域进行了超微结构研究。可见胆碱能纤维随睾丸动脉穿入白膜，在睾丸纵隔形成广泛的网络，许多纤维终止于睾丸网上皮下方。儿茶酚胺能纤维穿过附睾尾部中部，在覆盖睾丸下极的白膜中形成一个丛。该丛发出形成静脉曲张的直纤维，其中一些似乎与肥大细胞有关。仅在睾丸网上皮下方发现含肌动蛋白的细胞。这些细胞类似于肌成纤维细胞。胆碱能纤维和收缩细胞在睾丸网管道中的位置表明，这些细胞可能参与将精液泵入输出小管，并且它们的收缩性可能受胆碱能纤维调节。
