Yang Ding-wei, Lin Shan, Yang Ding-ping, Wei Li, Shang Wen-ya
Division of Nephrology, Department of Medicine, General Hospital of Tianjin medical University, Tianjin 300052, China. Email:
Zhonghua Yi Xue Za Zhi. 2012 May 29;92(20):1424-7.
To explore the effects of short- and long-term dietary hypercholesterolemia on contrast media-induced nephrotoxicity in rats.
The male Wistar rats were fed either a normal rodent diet or a high cholesterol diet. At the end of 2 and 8 weeks, 8 rats from each group received a tail vein injection of either Iohexol injection (groups NC and HC) or vehicle (groups N and H). Blood lipid, renal function, renal hemodynamics, renal and urinary prostaglandin E2 (PGE2) and thromboxane B2 (TXB2), renal nitric oxide and malondialdehyde (MDA) were determined at Day 1 following the administration of contrast media.
The dosing of contrast media induced obviously increased serum creatinine compared with normal rats ((185 ± 28) vs (53 ± 3) µmol/L, P < 0.01) and severe renal tubular necrosis in rats with a high cholesterol diet for 8 weeks but did not in normal-diet rats or rats with a high cholesterol diet for 2 weeks. The renal and urinary levels of PGE2 and TXB2 increased significantly in rats of groups H and HC at the end of 8 weeks. The renal production of nitric oxide decreased while the concentration of MDA increased markedly in groups HC and H at the end of 8 weeks.
Long-term hypercholesterolemia appears to be a risk factor of contrast media-induced acute renal failure. And it may be associated with the disorder of intrarenal prostaglandins and the abnormality of renal nitric oxide system as induced by lipid peroxidation.
探讨短期和长期饮食性高胆固醇血症对大鼠造影剂诱导的肾毒性的影响。
将雄性Wistar大鼠分为正常啮齿动物饮食组和高胆固醇饮食组。在2周和8周结束时，每组8只大鼠经尾静脉注射碘海醇注射液（NC组和HC组）或溶剂（N组和H组）。在给予造影剂后第1天测定血脂、肾功能、肾血流动力学、肾和尿前列腺素E2（PGE2）和血栓素B2（TXB2）、肾一氧化氮和丙二醛（MDA）。
与正常大鼠相比，造影剂给药后血清肌酐明显升高（(185 ± 28) 对 (53 ± 3) µmol/L，P < 0.01），且高胆固醇饮食8周的大鼠出现严重肾小管坏死，而正常饮食大鼠或高胆固醇饮食2周的大鼠未出现。在8周结束时，H组和HC组大鼠肾和尿中PGE2和TXB2水平显著升高。在8周结束时，HC组和H组大鼠肾一氧化氮生成减少而MDA浓度显著增加。
长期高胆固醇血症似乎是造影剂诱导的急性肾衰竭的一个危险因素。它可能与肾内前列腺素紊乱以及脂质过氧化诱导的肾一氧化氮系统异常有关。
