基质金属蛋白酶及基质金属蛋白酶组织抑制剂在非黑素瘤皮肤癌中的表达及意义

[Expression and significance of matrix metalloproteinase and tissue inhibitor of matrix metalloproteinase in non-melanoma skin cancer].

作者信息

Fu Xiao-rui, Zhang Chen, Chen Chang-ying, Zhang Lei, Wang Liu-xing, Wang Bian-hong, Liu Xi-yang, Zhang Ming-zhi

机构信息

Department of Oncology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

出版信息

Zhonghua Zhong Liu Za Zhi. 2012 May;34(5):369-73. doi: 10. 3760/cma.j.issn.0253-3766.2012.05.011.

DOI:10. 3760/cma.j.issn.0253-3766.2012.05.011

PMID:22883459

Abstract

OBJECTIVE

To investigate the expression and significance of matrix metalloproteinases (MMP-2, MMP-9) and tissue inhibitor of matrix metalloproteinase (TIMP-2, TIMP-1) in non-melanoma skin cancer (NMSC).

METHODS

Thirty six patients with squamous cell carcinoma (SCC) and 32 patients with basal cell carcinoma (BCC), confirmed by pathology, were selected, and 30 cases of normal skin were selected as control. The expression of MMP-2, MMP-9, TIMP-1 and TIMP-2 in all samples were examined by immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR). The expression rate, expression intensity and expression level of each factor were recorded. The results were compared between the groups.

RESULTS

The expression rates of MMP-2 and MMP-9 in the control group were 30.0% and 36.7%, the expression levels of MMP-2 and MMP-9 in the control group were 57.216 ± 12.785 and 59.318 ± 13.262, all significantly lower than those in the tumor edge and center of the SCC and BCC groups (P < 0.01). The expression rates of TIMP-1 and TIMP-2 in the control group were 96.7% and 100%, their expression levels were 121.738 ± 25.516 and 122.612 ± 25.964, all significantly higher than those in the SCC and BCC groups (P < 0.01). The expression levels of MMP-2 and MMP-9 in the tumor center and edge of SCC group were significantly higher than those in the corresponding parts of the BCC group, while the expression levels of TIMP-1 and TIMP-2 were significantly lower than those in the BCC group (P < 0.01). The expression levels of MMP-2 and MMP-9 in the tumor edge of the SCC and BCC groups were significantly higher than those in the tumor centers (P < 0.01), while the expression levels of TIMP-1and TIMP-2 were significantly lower than those in the tumor centers (P < 0.01).

CONCLUSION

MMP-2, MMP-9 and TIMP-2, TIMP-1 may play an important role in the development, progression, invasion and metastasis of non-melanoma skin cancer.

摘要

目的

探讨基质金属蛋白酶（MMP - 2、MMP - 9）及基质金属蛋白酶组织抑制剂（TIMP - 2、TIMP - 1）在非黑素瘤皮肤癌（NMSC）中的表达及意义。

方法

选取经病理确诊的36例鳞状细胞癌（SCC）患者和32例基底细胞癌（BCC）患者，另选30例正常皮肤作为对照。采用免疫组织化学和逆转录聚合酶链反应（RT - PCR）检测所有样本中MMP - 2、MMP - 9、TIMP - 1和TIMP - 2的表达。记录各因子的表达率、表达强度及表达水平，并进行组间比较。

结果

对照组中MMP - 2和MMP - 9的表达率分别为30.0%和36.7%，MMP - 2和MMP - 9的表达水平分别为57.216 ± 12.785和59.318 ± 13.262，均显著低于SCC组和BCC组肿瘤边缘及中心的表达（P < 0.01）。对照组中TIMP - 1和TIMP - 2的表达率分别为96.7%和100%，其表达水平分别为121.738 ± 25.516和122.612 ± 25.964，均显著高于SCC组和BCC组（P < 0.01）。SCC组肿瘤中心及边缘MMP - 2和MMP - 9的表达水平显著高于BCC组相应部位，而TIMP - 1和TIMP - 2的表达水平显著低于BCC组（P < 0.01）。SCC组和BCC组肿瘤边缘MMP - 2和MMP - 9的表达水平显著高于肿瘤中心（P < 0.01），而TIMP - 1和TIMP - 2的表达水平显著低于肿瘤中心（P < 0.01）。