Wang Wei, Lin Peng, Sun Bao-cun, Cai Wen-juan, Han Chun-rong, Li Li, Lu Hong-hua, Zhang Jin-mei
Department of Otorhinolaryngology Head and Neck Surgery, Tianjin First Central Hospital, Tianjin Otorhinolaryngology Institution, the First Clinical Department of Tianjin Medical University, Tianjin 300384, China.
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2012 May;47(5):400-5.
To investigate the contribution of vasculogenic mimicry (VM) and endothelium-dependent vessel (EDV) to invasion and metastasis of laryngeal squamous cell carcinoma (LSCC).
A total of 203 cases with LSCC was reviewed and followed up. VM and EDV in LSCC tissues were assessed by double staining with anti-CD31 immunohistochemistry and periodic acid-schiff. Kruskal-Wallis test and one-way ANOVA were used to analyze the relationship between VM, EDV and clinical pathology parameters of LSCC. Kaplan-Meier analysis was used to evaluate overall survival (OS) of patients with LSCC.
VM related to pTNM stage, lymph node metastasis and pathology grade of LSCC, while EDV related to primary sites, pTNM stage, T stage and distant metastasis of LSCC. Univariate analysis showed VM (P = 0.014), pTNM stage (P = 0.009), T stage (P = 0.013), nodal status (P = 0.013), histopathology grade (P = 0.038), tumor size (P = 0.028), and radiotherapy (P < 0.0001) related to OS. VM (P = 0.011), primary sites (P = 0.049), tumor size (P = 0.001) and radiotherapy (P < 0.0001) related to disease free survival. Multivariate analysis indicated that VM was an adverse predictor for both OS and disease free survival.
Both VM and EDV existed in LSCC. VM contributed to progression of LSCC through promoting lymph node metastasis. VM is an independent predictor for the prognosis of LSCC.
探讨血管生成拟态（VM）和内皮依赖性血管（EDV）在喉鳞状细胞癌（LSCC）侵袭和转移中的作用。
回顾性分析203例LSCC患者的临床资料并进行随访。采用抗CD31免疫组化和过碘酸-希夫双重染色法评估LSCC组织中的VM和EDV。采用Kruskal-Wallis检验和单因素方差分析分析VM、EDV与LSCC临床病理参数之间的关系。采用Kaplan-Meier分析评估LSCC患者的总生存期（OS）。
VM与LSCC的pTNM分期、淋巴结转移和病理分级有关，而EDV与LSCC的原发部位、pTNM分期、T分期和远处转移有关。单因素分析显示VM（P = 0.014）、pTNM分期（P = 0.009）、T分期（P = 0.013）、淋巴结状态（P = 0.013）、组织病理学分级（P = 0.038）、肿瘤大小（P = 0.028）和放疗（P < 0.0001）与OS有关。VM（P = 0.011）、原发部位（P = 0.049）、肿瘤大小（P = 0.001）和放疗（P < 0.0001）与无病生存期有关。多因素分析表明，VM是OS和无病生存期的不良预测指标。
LSCC中同时存在VM和EDV。VM通过促进淋巴结转移促进LSCC进展。VM是LSCC预后的独立预测指标。
