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血管紧张素 III 和血管紧张素 II 对人前列腺癌细胞迁移和增殖的影响的异同。

Similarities and differences between effects of angiotensin III and angiotensin II on human prostate cancer cell migration and proliferation.

机构信息

Department of Comparative Endocrinology, Medical University of Lodz, Poland.

出版信息

Peptides. 2012 Oct;37(2):200-6. doi: 10.1016/j.peptides.2012.07.022. Epub 2012 Aug 3.

Abstract

Proliferation plays a critical role in tumor growth when cell migration is essential to invasion. The effect of Ang III and Ang II was evaluated on these important processes. Changes in the migration potential of prostate cancer cells were investigated using Wound Healing Test and a Transwell Migration Chamber with a 3 μm pore size. Cell proliferation was measured with a BrdU Assay and Countess Automated Cell Counter, thus determining the influence of angiotensins on hormone-dependent (LNCaP) and hormone-independent (DU-145) human prostate cancer lines. The influence of Ang III and Ang II on classic receptors may be inhibited by Losartan or PD123319. Test peptide modulation of the AT1 and AT2 receptors was examined by Western Blot and fluorescent immunocytochemistry. The results indicate that Ang III promotes the migration of both LNCaP and DU-145 lines, whereas Ang II stimulates this process only in androgen-independent cells. Both angiotensin peptides can induce prostate cancer cell proliferation in a time- and dose-dependent manner. The obtained results show that Ang III and Ang II can modify the expression of classic receptors, particularly AT2. These results suggest that the investigated peptide can modulate cell migration and proliferation in prostate cancer cells. Angiotensins probably have a greater influence on proliferation in the early-stage prostate cancer model than hormone-independent cell lines. Assume also that Ang II can enhance the migration tendency aggressive prostate cancer cells, while Ang III does so more effective in non-metastatic cells.

摘要

增殖在肿瘤生长中起着关键作用,当细胞迁移对侵袭至关重要时。评估了 Ang III 和 Ang II 对这些重要过程的影响。使用划痕愈合试验和具有 3 μm 孔径的 Transwell 迁移室研究了前列腺癌细胞迁移潜力的变化。通过 BrdU 检测和 Countess 自动化细胞计数器测量细胞增殖,从而确定血管紧张素对激素依赖性(LNCaP)和激素非依赖性(DU-145)人前列腺癌细胞系的影响。Ang III 和 Ang II 对经典受体的影响可能被 Losartan 或 PD123319 抑制。通过 Western Blot 和荧光免疫细胞化学检查了测试肽对 AT1 和 AT2 受体的调节作用。结果表明,Ang III 促进了 LNCaP 和 DU-145 系的迁移,而 Ang II 仅在雄激素非依赖性细胞中刺激该过程。两种血管紧张素肽都可以以时间和剂量依赖的方式诱导前列腺癌细胞增殖。所得结果表明,Ang III 和 Ang II 可以修饰经典受体的表达,特别是 AT2。这些结果表明,研究肽可以调节前列腺癌细胞的迁移和增殖。血管紧张素可能对早期前列腺癌模型中的增殖有更大的影响,而不是激素非依赖性细胞系。还假设 Ang II 可以增强侵袭性前列腺癌细胞的迁移趋势,而 Ang III 在非转移性细胞中更有效。

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