Biological and Environmental Sciences, School of Natural Sciences, University of Stirling, Stirling FK9 4LA, Scotland, UK.
Comp Biochem Physiol B Biochem Mol Biol. 2012 Nov-Dec;163(3-4):303-8. doi: 10.1016/j.cbpb.2012.07.003. Epub 2012 Aug 3.
Inhibitors of phenoloxidase are used routinely to characterise the structural and functional properties of phenoloxidases. Hemocyanin-derived phenoloxidase activity is also sensitive to standard phenoloxidase inhibitors. In this study, we characterise the effects of a number of phenoloxidase inhibitors on hemocyanin-derived phenoloxidase activity from the chelicerate, Limulus polyphemus. Both inhibition type and K(i) values were similar to those observed for hemocyanin-derived phenoloxidase from another chelicerate, Eurypelma californicum. In addition, substrate inhibition was observed at concentrations above 2mM dopamine. The conformation in which two of the inhibitors, namely tropolone and kojic acid, would bind near the Cu(II) centre of hemocyanin is proposed.
抑制剂的酚氧化酶被常规用于描述酚氧化酶的结构和功能特性。血蓝蛋白衍生的酚氧化酶活性也对标准酚氧化酶抑制剂敏感。在这项研究中，我们描述了一些酚氧化酶抑制剂对节肢动物鲎血蓝蛋白衍生的酚氧化酶活性的影响。抑制类型和 K(i) 值与另一种节肢动物加利福尼亚州 Eurypelma californicum 的血蓝蛋白衍生的酚氧化酶相似。此外，在多巴胺浓度高于 2mM 时观察到底物抑制。提出了抑制剂之一曲通和曲酸在靠近血蓝蛋白的 Cu(II) 中心结合的构象。
