Tien H T, Salamon Z
Department of Physiology, Michigan State University, East Lansing 48824.
Biotechnol Appl Biochem. 1990 Oct;12(5):478-84.
Solid-supported bilayer lipid membranes (s-BLMs) that possess some properties similar to those of conventional BLMs can be self-assembled on a freshly cleaved metal wire by a two-step procedure: (i) The tip of a Teflon-coated platinum wire, while immersed in a lipid solution, is cut off with a scalpel; (ii) the new tip of the wire, having become coated with lipid solution, is transferred into 0.1 M KCl. After a few minutes, a stable lipid bilayer forms spontaneously on the tip of the wire, as verified by electrical measurements. An application of such a supported BLM (s-BLM) is reported for the detection of Pb2+ ions. The s-BLM is liquid-crystalline in structure, which makes it amenable to modification for basic studies, as well as for technological applications such as biosensors and molecular electronic devices.
具有一些与传统双层脂质膜（BLMs）相似性质的固体支撑双层脂质膜（s-BLMs）可以通过两步法在新切割的金属丝上自组装：（i）将涂有特氟龙的铂丝尖端浸入脂质溶液中，用手术刀切断；（ii）已被脂质溶液覆盖的金属丝新尖端转移到0.1 M KCl中。几分钟后，如电学测量所证实的，金属丝尖端会自发形成稳定的脂质双层。报道了这种支撑双层脂质膜（s-BLM）在检测Pb2+离子方面的应用。s-BLM结构为液晶态，这使其适合用于基础研究的修饰，以及诸如生物传感器和分子电子器件等技术应用。
