CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
Electrophoresis. 2012 Aug;33(15):2399-406. doi: 10.1002/elps.201100581.
In this work a chip-based nano HPLC coupled MS (HPLC-chip/MS) method with a simple sample preparation procedure was developed for the flavonoid profiling of soybean. The analytical properties of the method including the linearity (R(2) , 0.992-0.995), reproducibility (RSD, 1.50-7.66%), intraday precision (RSD, 1.41-5.14%) and interday precision (RSD, 2.76-16.90%) were satisfactory. Compared with the conventional HPLC/MS method, a fast extraction and analysis procedure was applied and more flavonoids were detected in a single run. Additionally, 13 flavonoids in soybean seed were identified for the first time. The method was then applied to the profiling of six varieties of soybean sowed at the same place. A clear discrimination was observed among different cultivars, three isoflavones, accounting for nearly 80% of total flavonoid contents, were found increased in the spring soybeans compared with the summer cultivars.
本工作建立了一种基于芯片的纳升级高效液相色谱-串联质谱（HPLC-chip/MS）方法，用于大豆中类黄酮的分析。该方法具有简单的样品制备程序，分析性能良好，包括线性（R²，0.992-0.995）、重现性（RSD，1.50-7.66%）、日内精密度（RSD，1.41-5.14%）和日间精密度（RSD，2.76-16.90%）。与传统的 HPLC/MS 方法相比，该方法应用了快速的提取和分析程序，在单次运行中可检测到更多的类黄酮。此外，首次鉴定了大豆种子中的 13 种类黄酮。该方法随后应用于同一地点种植的 6 个大豆品种的分析。不同品种之间存在明显的差异，春大豆中三种异黄酮的含量占总类黄酮含量的近 80%，明显高于夏大豆品种。
