尿脱落细胞学中细胞角蛋白-20免疫细胞化学及其在检测尿路上皮癌中与核基质蛋白-22和尿细胞学的比较。

Cytokeratin-20 immunocytochemistry in voided urine cytology and its comparison with nuclear matrix protein-22 and urine cytology in the detection of urothelial carcinoma.

作者信息

Srivastava Ruchi, Arora Vinod Kumar, Aggarwal Seema, Bhatia Arati, Singh Navjeevan, Agrawal Vivek

机构信息

Department of Pathology, University College of Medical Sciences and Guru Teg Bahadur Hospital, Dilshad Garden, Delhi, India.

出版信息

Diagn Cytopathol. 2012 Sep;40(9):755-9. doi: 10.1002/dc.21617. Epub 2011 Jan 6.

DOI:10.1002/dc.21617

PMID:22888081

Abstract

This study was done on 59 subjects (42 urinary bladder carcinoma patients and 17 non-neoplastic controls). Urine cytology and bladder chek NMP22 test was done on all cases. CK20 immunostaining was performed on archived papanicolaou stained urine cytology smears in 34 cases (27 bladder carcinoma and 7 negative controls). Results of all three tests (cytology, NMP22, and CK20 immunostaining) were compared with histopathology to evaluate the accuracy of individual test. The combination of cytology and NMP22 was compared with combination of cytology and CK20 immunostaining for detection of bladder carcinoma. NMP22 had sensitivity of 92.9% and specificity of 70.6%, as compared with voided urine cytology (sensitivity of 76.2% and specificity of 76.5%) and CK20 immunostaining (sensitivity of 70.4% and specificity of 71.4%). Combination of cytology and NMP22 gave better results (sensitivity of 88.1% and specificity of 88.2%) than combination of cytology and CK20 immunostaining or any other test in isolation.

摘要

本研究针对59名受试者（42例膀胱癌患者和17名非肿瘤对照者）开展。对所有病例均进行了尿细胞学检查及膀胱Chek NMP22检测。对34例病例（27例膀胱癌和7例阴性对照）存档的巴氏染色尿细胞学涂片进行了细胞角蛋白20（CK20）免疫染色。将三项检测（细胞学检查、NMP22检测和CK20免疫染色）的结果与组织病理学结果进行比较，以评估各项检测的准确性。比较了细胞学检查与NMP22联合检测和细胞学检查与CK20免疫染色联合检测对膀胱癌的诊断效能。与尿脱落细胞学检查（敏感性76.2%，特异性76.5%）及CK20免疫染色（敏感性70.4%，特异性71.4%）相比，NMP22的敏感性为92.9%，特异性为70.6%。细胞学检查与NMP22联合检测的结果（敏感性88.1%，特异性88.2%）优于细胞学检查与CK20免疫染色联合检测或任何一项单独检测。