Department of Chemical and Biomolecular Engineering, The University of Melbourne, Melbourne, Victoria 3010, Australia.
Langmuir. 2012 Aug 28;28(34):12527-35. doi: 10.1021/la302587t. Epub 2012 Aug 13.
DNA films are of interest for use in a number of areas, including sensing, diagnostics, and as drug/gene delivery carriers. The specific base pairing of DNA materials can be used to manipulate their architecture and degradability. The programmable nature of these materials leads to complex and unexpected structures that can be formed from solution assembly. Herein, we investigate the structure of DNA multilayer films using Förster resonance energy transfer (FRET). The DNA films are assembled on silica particles by depositing alternating layers of homopolymeric diblocks (polyA(15)G(15) and polyT(15)C(15)) with fluorophore (polyA(15)G(15)-TAMRA) and quencher (polyT(15)C(15)-BHQ2) layers incorporated at predesigned locations throughout the films. Our results show that DNA films are dynamic structures that undergo rearrangement. This occurs when the multilayer films are perturbed during new layer formation through hybridization but can also take place spontaneously when left over time. These films are anticipated to be useful in drug delivery applications and sensing applications.
DNA 薄膜在多个领域都有应用，包括传感、诊断和药物/基因传递载体。DNA 材料的特定碱基配对可用于操纵其结构和降解性。这些材料的可编程性质导致了可以从溶液组装形成的复杂和意想不到的结构。在此，我们使用Förster 共振能量转移（FRET）研究 DNA 多层膜的结构。通过在二氧化硅颗粒上沉积具有荧光团（polyA(15)G(15)-TAMRA）和猝灭剂（polyT(15)C(15)-BHQ2）层的均聚物二嵌段（polyA(15)G(15)和 polyT(15)C(15)）的交替层，将 DNA 薄膜组装在二氧化硅颗粒上，荧光团和猝灭剂层被预先设计的位置嵌入整个薄膜中。我们的结果表明，DNA 薄膜是经历重排的动态结构。当多层膜在新层形成过程中通过杂交受到干扰时会发生这种情况，但随着时间的推移也会自发发生。这些薄膜有望在药物传递应用和传感应用中有用。
