Departments of Endodontics and Basic Sciences, Dental School, University of Athens, Athens, Greece.
Int Endod J. 2013 Feb;46(2):160-8. doi: 10.1111/j.1365-2591.2012.02103.x. Epub 2012 Aug 13.
To investigate the expression of two endoplasmic reticulum (ER)-resident key chaperone proteins, ERdj5 and BiP, under the influence of resinous monomers and its relationship with the inhibition of mineralization caused by the monomer 2-hydroxyethyl methacrylate (HEMA).
The ERdj5 and BiP expression was studied in vitro, in primary human pulp cell cultures after treatment with three different HEMA concentrations at different time periods. Subsequently, the expression of both the odontoblast markers dentine sialoprotein (DSP) and osteonectin (OSN) was studied in human pulp cells under the same conditions.
The ERdj5 and BiP expression was upregulated in the pulp cells. DSP and OSN were largely dispersed in the cytoplasm in control cell cultures but accumulated in a perinuclear area after exposure to HEMA. Their expression levels were not affected.
The increased expression of ERdj5 and BiP may reflect activation of ER stress. DSP and OSN accumulation into the cells may lead to their secretion arrest and inhibition of dentine matrix formation. These events may elucidate the mechanism by which HEMA inhibits the mineralization process.
研究内质网（ER）驻留的两种关键伴侣蛋白 ERdj5 和 BiP 在树脂单体影响下的表达情况及其与单体 2-羟乙基甲基丙烯酸酯（HEMA）抑制矿化的关系。
体外培养原代人牙髓细胞，用三种不同浓度的 HEMA 处理不同时间，研究 ERdj5 和 BiP 的表达。随后，在相同条件下研究牙髓细胞中两种成牙本质细胞标志物牙本质涎磷蛋白（DSP）和骨桥蛋白（OSN）的表达。
牙髓细胞中 ERdj5 和 BiP 的表达上调。在对照细胞培养物中，DSP 和 OSN 主要分布在细胞质中，但暴露于 HEMA 后会聚集在核周区。它们的表达水平没有受到影响。
ERdj5 和 BiP 的表达增加可能反映了内质网应激的激活。DSP 和 OSN 积累到细胞中可能导致其分泌受阻，抑制牙本质基质的形成。这些事件可能阐明了 HEMA 抑制矿化过程的机制。
