Centro de Química Medicinal da, Universidade do Porto (CEQUIMED-UP), Rua de Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal.
J Chromatogr A. 2012 Dec 21;1269:143-53. doi: 10.1016/j.chroma.2012.07.058. Epub 2012 Jul 25.
The enantioresolution and determination of the enantiomeric purity of 24 new chiral derivatives of xanthones (CDXs) were investigated on polysaccharide-based chiral stationary phases (CSPs). The tris-3,5-dimethylphenylcarbamates of cellulose and amylose and tris-3,5-dimethoxyphenylcarbamate of amylose were selected as CSPs for this work. The separation of enantiomeric mixtures of CDXs was explored under multimodal elution conditions. All the enantiomeric mixtures of CDXs were enantioseparated with very high enantioselectivity and resolution with α and R(S) ranging from 1.43 to 12.41 and from 1.48 to 10.29, respectively. The best performances were achieved on amylose tris-3,5-dimethylphenylcarbamate stationary phase under polar organic elution conditions. Furthermore the enantiomeric purity for all the CDXs was measured, achieving values higher than 99%. Based on the obtained results, the influence of the mobile phases and structural features of the CSPs and CDXs on chiral discrimination are also discussed.
研究了手性固定相（CSP）上 24 种新型黄烷酮（CDX）手性衍生物的对映体拆分和对映体纯度的测定。选择纤维素、淀粉的三-3,5-二甲基苯甲酰基和淀粉的三-3,5-二甲氧基苯甲酰基作为 CSP。在多模态洗脱条件下探索了 CDX 对映体混合物的分离。所有 CDX 的对映体混合物都具有非常高的对映选择性和分辨率，α和 R(S)值分别为 1.43 至 12.41 和 1.48 至 10.29。在极性有机洗脱条件下，淀粉三-3,5-二甲基苯甲酰基固定相的性能最佳。此外，还测量了所有 CDX 的对映体纯度，达到了 99%以上。基于所获得的结果，还讨论了流动相和 CSP 和 CDX 的结构特征对手性识别的影响。
