Laboratory for Chemical Protein Synthesis, Department of Biochemistry, Maastricht University, Universiteitssingel 50, 6200 MD, Maastricht, The Netherlands.
Chem Commun (Camb). 2012 Sep 28;48(75):9403-5. doi: 10.1039/c2cc34956f. Epub 2012 Aug 13.
The use of omonasteine (Omo) in sequential peptide ligation strategies extends the scope of homocysteine (Hcy) ligation to longer, methionine-rich proteins. Hcy-to-Omo conversion can be performed on-resin, while the Omo-to-Hcy deprotection can be performed in situ after peptide ligation. This strategy was successfully applied in the synthesis of the BRD7 bromodomain.
在序列肽连接策略中使用奥莫那肽（Omo）将同型半胱氨酸（Hcy）连接的范围扩展到更长的富含蛋氨酸的蛋白质。Hcy 到 Omo 的转化可以在树脂上进行，而 Omo 到 Hcy 的脱保护可以在肽连接后就地进行。该策略成功应用于 BRD7 溴结构域的合成。
