Young K H, Kraeling R R, Bazer F W
Animal Science Department, University of Florida, Gainesville 32611.
Biol Reprod. 1990 Oct;43(4):592-9. doi: 10.1095/biolreprod43.4.592.
During gestation, pigs have constant circulating levels of prolactin (PRL), and lack decidual PRL and placental lactogens. Effects of PRL on uterine physiology in pigs may be due to changes in endometrial PRL receptors. In this study, effects of the conceptus and cyclic hormonal environment on endometrial PRL receptors were investigated. Endometrial PRL receptor numbers were similar between Days 8 and 15 for cyclic gilts. In contrast, endometrial PRL receptor numbers for pregnant gilts were similar between Days 8 and 11, then increased (p less than 0.05) on Day 12 and remained elevated between Days 14 and 30. This day-by-status interaction approached significance (p less than 0.06) and overall receptor numbers were higher (p less than 0.01) for pregnant than for cyclic gilts. Pig conceptuses secrete estrogen between Days 11 and 12; therefore, regulation of endometrial PRL receptors by acute administration of estradiol was investigated. Uterine flushings and endometrium were collected from one uterine horn of cyclic gilts on Day 11; then, at 1, 6, 12, and 24 h following a single injection of estradiol valerate (EV; 5 mg, into adipose tissue), uterine flushings and endometrium were collected from the second uterine horn. Endometrial PRL receptor numbers were higher (p less than 0.05) at both 1 h and 6 h after treatment with EV and then decreased (p less than 0.02) by 12 h to below pretreatment values. In uterine flushings, total recoverable protein (p less than 0.05), uteroferrin (p less than 0.01), leucine aminopeptidase (p less than 0.05), calcium (p less than 0.03), sodium (p less than 0.01), and potassium (p less than 0.05) increased between 12 and 24 h following EV treatment.(ABSTRACT TRUNCATED AT 250 WORDS)
在妊娠期，母猪的催乳素（PRL）循环水平恒定，且缺乏蜕膜催乳素和胎盘催乳素。催乳素对母猪子宫生理的影响可能归因于子宫内膜催乳素受体的变化。在本研究中，研究了孕体和周期性激素环境对子宫内膜催乳素受体的影响。对于发情周期的后备母猪，在第8天至第15天期间，子宫内膜催乳素受体数量相似。相比之下，妊娠母猪的子宫内膜催乳素受体数量在第8天至第11天相似，然后在第12天增加（p<0.05），并在第14天至第30天保持升高。这种按状态划分的天数交互作用接近显著水平（p<0.06），并且妊娠母猪的总体受体数量高于发情周期的后备母猪（p<0.01）。母猪孕体在第11天至第12天分泌雌激素；因此，研究了通过急性给予雌二醇对子宫内膜催乳素受体的调节作用。在第11天从发情周期后备母猪的一个子宫角收集子宫冲洗液和子宫内膜；然后，在单次注射戊酸雌二醇（EV；5mg，注入脂肪组织）后的1、6、12和24小时，从第二个子宫角收集子宫冲洗液和子宫内膜。用EV处理后1小时和6小时，子宫内膜催乳素受体数量均较高（p<0.05），然后在12小时时下降（p<0.02）至预处理值以下。在子宫冲洗液中，EV处理后12至24小时，总可回收蛋白（p<0.05）、子宫铁蛋白（p<0.01）、亮氨酸氨肽酶（p<0.05）、钙（p<0.03）、钠（p<0.01）和钾（p<0.05）增加。（摘要截于250字）
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