Young K H, Kraeling R R, Bazer F W
Animal Science Department, University of Florida, Gainesville 32611.
J Reprod Fertil. 1989 Jul;86(2):713-22. doi: 10.1530/jrf.0.0860713.
Hypoprolactinaemia was induced by bromocriptine (CB154; 100 mg/day) which decreased circulating prolactin by 40% (P less than 0.06), but did not affect conceptus survival at Day 25 when administered on Days 10-16 when compared to saline:ethanol-treated control gilts. Bromocriptine or vehicle was administered to cyclic gilts on Days 10-11, oestradiol valerate was injected on Day 11 and uterine flushings were collected on Day 12. Total recoverable protein and uteroferrin in uterine flushings were not affected by treatment. However, leucine aminopeptidase activity (P less than 0.02) and total recoverable Ca2+, Na+, K+ and Cl- (P less than 0.05) were decreased in uterine flushings of gilts that received bromocriptine, suggesting that hypoprolactinaemia decreased general secretory activity of the endometrial epithelium and modulated ionic changes, respectively, in the uterine environment of pigs. Subcutaneous administration of pig prolactin (1 mg/12 h) increased (P less than 0.001) serum prolactin 4.5-fold. The interaction between hyperprolactinaemia and progesterone, without oestrogen, on components of uterine flushings were determined using gilts that received progesterone (200 mg/day) and prolactin or saline on Days 4-14 after ovariectomy on Day 4. On Day 15, there were no differences (P greater than 0.05) in any of the uterine secretory components measured. Hyperprolactinaemia (1 mg pig prolactin on Days 6-11) enhanced overall uterine secretory response on Day 12 to oestradiol (5 mg) administered on Day 11 compared to gilts that received 1 ml saline on Days 6-11 of the oestrous cycle. Total recoverable protein and leucine aminopeptidase activity were greater (P less than 0.05) for oestradiol-treated gilts, but effects of prolactin were not significant. Total recoverable glucose (P less than 0.01), PGF-2 alpha (P less than 0.02), uteroferrin (P less than 0.01) and specific activity of uteroferrin (P less than 0.001) were increased by prolactin and oestradiol, but not oestradiol alone. Calcium (P less than 0.05), chloride (P less than 0.05) and potassium (P less than 0.01) were increased in response to oestradiol. These results indicate an interaction between oestradiol and prolactin, but not progesterone and prolactin, which enhances secretion of some products of the pig uterine endometrium.
用溴隐亭（CB154；100毫克/天）诱发低催乳素血症，其使循环催乳素水平降低了40%（P<0.06），但在第10 - 16天给药时，与生理盐水：乙醇处理的对照后备母猪相比，在第25天时对胚胎存活没有影响。在第10 - 11天给处于发情周期的后备母猪注射溴隐亭或赋形剂，在第11天注射戊酸雌二醇，并在第12天收集子宫冲洗液。处理对子宫冲洗液中总可回收蛋白和子宫铁蛋白没有影响。然而，接受溴隐亭的后备母猪子宫冲洗液中的亮氨酸氨肽酶活性（P<0.02）以及总可回收的钙、钠、钾和氯（P<0.05）降低，这表明低催乳素血症分别降低了子宫内膜上皮的一般分泌活性并调节了猪子宫环境中的离子变化。皮下注射猪催乳素（1毫克/12小时）使血清催乳素升高了4.5倍（P<0.001）。使用在第4天卵巢切除术后第4 - 14天接受孕酮（200毫克/天）和催乳素或生理盐水的后备母猪，确定了高催乳素血症与无雌激素的孕酮对子宫冲洗液成分的相互作用。在第15天，所测量的任何子宫分泌成分均无差异（P>0.05）。与在发情周期第6 - 11天接受1毫升生理盐水的后备母猪相比，高催乳素血症（在第6 - 11天注射1毫克猪催乳素）增强了第12天子宫对第11天注射的雌二醇（5毫克）的总体分泌反应。雌二醇处理的后备母猪的总可回收蛋白和亮氨酸氨肽酶活性更高（P<0.05），但催乳素的影响不显著。催乳素和雌二醇可增加总可回收葡萄糖（P<0.01）、前列腺素F - 2α（P<0.02）、子宫铁蛋白（P<0.01）和子宫铁蛋白的比活性（P<0.001），但单独使用雌二醇则无此作用。钙（P<0.05）、氯（P<0.05）和钾（P<0.01）对雌二醇有反应而增加。这些结果表明雌二醇与催乳素之间存在相互作用，但孕酮与催乳素之间不存在这种相互作用，这种相互作用增强了猪子宫内膜某些产物的分泌。
