Department of Pharmaceutics, Poona College of Pharmacy, Bharati Vidyapeeth University, Erandwane, Pune 411038, India.
Int J Pharm. 2012 Nov 1;437(1-2):29-41. doi: 10.1016/j.ijpharm.2012.07.062. Epub 2012 Aug 4.
Curcumin, a natural phytoconstituent, is known to be therapeutically effective in the treatment of various cancers such as, breast cancer, lung cancer, pancreatic cancer, brain cancer, etc. However, low bioavailability and photodegradation of curcumin hampers its overall therapeutic efficacy. Anionic polymerization method was employed for the preparation of apolipoprotein-E3 mediated curcumin loaded poly(butyl)cyanoacrylate nanoparticles (ApoE3-C-PBCA) and characterized for size, zeta potential, entrapment efficiency, photostability, morphology, and in vitro release study. ApoE3-C-PBCA were found to be effective against SH-SY5Y neuroblastoma cells compared to curcumin solution (CSSS) and curcumin loaded PBCA nanoparticles (C-PBCA) from in vitro cell culture investigations. Flow cytometry techniques employed for the detection of anticancer activity revealed enhanced activity of curcumin against SH-SY5Y neuroblastoma cells with ApoE3-C-PBCA compared to CSSS and C-PBCA, and apoptosis being the underlying mechanism. Present study revealed that ApoE3-C-PBCA has tremendous potential to develop into an effective therapeutic treatment modality against brain cancer.
姜黄素是一种天然植物成分，已被证实对多种癌症（如乳腺癌、肺癌、胰腺癌、脑癌等）具有治疗作用。然而，姜黄素的生物利用度低和光降解会影响其整体治疗效果。本研究采用阴离子聚合方法制备载姜黄素的载脂蛋白 E3 修饰的聚（正丁基）氰基丙烯酸酯纳米粒（ApoE3-C-PBCA），并对其粒径、Zeta 电位、包封率、光稳定性、形态和体外释放进行了表征。与姜黄素溶液（CSSS）和载姜黄素的聚（正丁基）氰基丙烯酸酯纳米粒（C-PBCA）相比，ApoE3-C-PBCA 对 SH-SY5Y 神经母细胞瘤细胞具有更好的抑制作用。通过流式细胞术检测抗癌活性的结果表明，与 CSSS 和 C-PBCA 相比，ApoE3-C-PBCA 使姜黄素对 SH-SY5Y 神经母细胞瘤细胞的活性增强，其作用机制可能与细胞凋亡有关。本研究表明，ApoE3-C-PBCA 具有巨大的潜力，可开发成为一种有效的脑癌治疗方法。
