Perrett D, Simmonds H A
Department of Medicine, St. Bartholomew's Hospital Medical College, West Smithfield, London, UK.
Biomed Chromatogr. 1990 Nov;4(6):267-72. doi: 10.1002/bmc.1130040613.
Purines and pyrimidines are now routinely separated by HPLC. By careful selection of chromatographic conditions which match the expected changes in hydrophobicity and/or ionic nature of the substrate and products most enzymes of the purine and pyrimidine salvage pathways can be routinely and accurately determined. Ion-paired reversed-phase systems are often the most advantageous. The relevance of such assays to biomedical analysis including their role in the diagnosis of inborn errors of metabolism is stressed.
目前，嘌呤和嘧啶通常通过高效液相色谱法进行分离。通过仔细选择与底物和产物预期的疏水性和/或离子性质变化相匹配的色谱条件，嘌呤和嘧啶补救途径的大多数酶都可以常规且准确地测定。离子对反相系统通常是最具优势的。强调了此类测定在生物医学分析中的相关性，包括它们在先天性代谢缺陷诊断中的作用。
