Graduate Group in Biological Engineering & Small-scale Technologies, University of California, Merced, Merced, CA 95343, USA.
Stem Cell Rev Rep. 2012 Dec;8(4):1120-8. doi: 10.1007/s12015-012-9403-6.
The generation of cardiomyocytes from human embryonic stem cells (hESC) boasts a variety of potential applications including cell transplantation for myocardial repair. Unfortunately, advancements in the field has been challenged by the low efficiency of cardiomyocyte differentiation from hESC. Recently, Kattman et al. 2011 showed that individual hESC lines require a precise balance of the Activin A and BMP4 signaling for efficient cardiac differentiation. This group also presented differentiation protocols for several human and mouse ESC lines, however; two of the most utilized hESC lines, the H9 and H7 ESC, were not included. Here, we provide protocols, based on the work from Kattman et al. 2011, for generating cardiomyoctyes from H7 and H9 hESC. These hESC line-specific protocols reproducibly direct approximately 50% of hESC towards the cardiac lineage.
从人类胚胎干细胞（hESC）中生成心肌细胞具有多种潜在应用，包括用于心肌修复的细胞移植。不幸的是，该领域的进展受到从 hESC 中生成心肌细胞效率低的挑战。最近，Kattman 等人。2011 年表明，单个 hESC 系需要精确平衡激活素 A 和 BMP4 信号以实现有效的心脏分化。该小组还提出了几种人类和小鼠 ESC 系的分化方案；然而，其中两个最常用的 hESC 系，H9 和 H7 ESC，并未包括在内。在这里，我们提供了基于 Kattman 等人的工作的方案。2011 年，用于从 H7 和 H9 hESC 生成心肌细胞。这些 hESC 系特异性方案可重复性地将大约 50%的 hESC 定向到心脏谱系。
