Gur-Cohen Shiri, Lapid Kfir, Lapidot Tsvee
Department of Immunology, Weizmann Institute of Science, Rehovot, Israel.
Methods Mol Biol. 2012;904:15-35. doi: 10.1007/978-1-61779-943-3_2.
Allogeneic donor blood cells and autologous peripheral blood leukocytes (PBL), obtained following -clinical mobilization procedures, are routinely used as a major source of hematopoietic stem and progenitor cells (HSPC) for transplantation protocols. It is, therefore, essential to evaluate and to quantify the extent by which the HSPC are mobilized and enriched in the circulation in correlation with their long-term hematopoietic reconstitution capacity. In this chapter, we describe quantitative methods that measure the number of mobilized HSPC according to specific criteria, as well as their functional properties in vitro and in vivo. The described assays are useful for assessment of progenitor cell mobilization as applied to both human and murine HSPC.
通过临床动员程序获得的异基因供体血细胞和自体外周血白细胞（PBL），通常用作移植方案中造血干细胞和祖细胞（HSPC）的主要来源。因此，评估和量化HSPC在循环中动员和富集的程度及其与长期造血重建能力的相关性至关重要。在本章中，我们描述了根据特定标准测量动员的HSPC数量的定量方法，以及它们在体外和体内的功能特性。所描述的测定方法对于评估应用于人类和小鼠HSPC的祖细胞动员很有用。
