Laboratory of Sustainable Technologies; UNAM Institute of Materials Science and Nanotechnology, Bilkent University, Ankara, Turkey.
Bioengineered. 2012 Nov-Dec;3(6):343-6. doi: 10.4161/bioe.21427. Epub 2012 Aug 15.
Nitrogen (N) and sulfur (S) have inter-related and distinct impacts on microalgal metabolism; with N starvation having previously been reported to induce elevated levels of the biodiesel feedstock material triacylglycerol (TAG), while S deprivation is extensively studied for its effects on biohydrogen production in microalgae. ( 1) (,) ( 2) We have previously demonstrated that N- and S-starved cells of Chlamydomonas reinhardtii display different metabolic trends, suggesting that different response mechanisms exist to compensate for the absence of those two elements. ( 3) We used C. reinhardtii CC-124 mt(-) and CC-125 mt(+) strains to test possible metabolic changes related to TAG accumulation in response to N and S deprivation, considering that gamete differentiation in this organism is mainly regulated by N. ( 4) Our findings contribute to the understanding of microalgal response to element deprivation and potential use of element deprivation for biodiesel feedstock production using microalgae, but much remains to be elucidated on the precise contribution of both N and S starvation on microalgal metabolism.
氮（N）和硫（S）对微藻代谢有相互关联和不同的影响；以前有报道称，氮饥饿会诱导生物柴油原料三酰基甘油（TAG）的水平升高，而 S 缺乏则被广泛研究用于研究其对微藻生物制氢的影响。(1) (，) (2) 我们之前已经证明，氮饥饿和硫饥饿的莱茵衣藻（Chlamydomonas reinhardtii）细胞表现出不同的代谢趋势，这表明存在不同的响应机制来补偿这两种元素的缺乏。(3) 我们使用莱茵衣藻 CC-124 mt(-)和 CC-125 mt(+)菌株来测试可能与 TAG 积累有关的代谢变化，以响应氮饥饿和硫饥饿，因为在该生物中配子分化主要由氮调节。(4) 我们的研究结果有助于理解微藻对元素缺乏的反应以及利用微藻进行生物柴油原料生产的元素缺乏的潜力，但关于氮饥饿和硫饥饿对微藻代谢的精确贡献仍有许多需要阐明。
