University of Cagliari, Department of Pathology, Cagliari, Italy.
Clinics (Sao Paulo). 2012 Jul;67(7):821-6. doi: 10.6061/clinics/2012(07)19.
The evaluation of S100B protein expression in the human heart and its correlation with drug-related death.
Left ventricular samples were collected from 74 serial forensic autopsies (15 overdose-related deaths; 59 non-overdose-related deaths) from 2007 to 2010. Tissue sections from each sample were immunostained for S100B protein by a commercial antibody.
The S100B protein was detected in the heart samples of all 15 cases of drug-related deaths; S100B immunoreactivity was mainly observed in the cytoplasm of cardiomyocytes and as globular deposits in the interstitial spaces. No reactivity or weak reactivity was found in the cardiomyocytes of the 59 subjects who died of other causes.
Our preliminary data show that the S100B protein accumulates in injured cardiomyocytes during drug-related sudden death. Given the near absence of S100B protein in the heart of subjects who died from causes other than drug overdose, S100B immunopositivity may be used as a new ancillary screening tool for the postmortem diagnosis of overdose-related cardiac death.
评估 S100B 蛋白在人心肌中的表达及其与药物相关死亡的相关性。
从 2007 年至 2010 年的 74 例连续法医解剖中收集左心室样本（15 例与药物过量相关的死亡；59 例与药物过量无关的死亡）。用商业抗体对每个样本的组织切片进行 S100B 蛋白免疫染色。
在所有 15 例与药物相关的死亡病例的心脏样本中均检测到 S100B 蛋白；S100B 免疫反应性主要观察到在心肌细胞的细胞质中和间质中的球状沉积物中。在死于其他原因的 59 名受试者的心肌细胞中未发现反应性或弱反应性。
我们的初步数据表明，S100B 蛋白在与药物相关的猝死期间在心肌细胞中积累。鉴于在因药物过量以外的原因死亡的受试者心脏中几乎不存在 S100B 蛋白，S100B 免疫阳性可能被用作药物过量相关心脏死亡的死后诊断的新辅助筛选工具。
