School of Chemistry, University of Southampton, Highfield, Southampton SO17 1BJ, UK.
Chem Commun (Camb). 2012 Nov 25;48(91):11184-6. doi: 10.1039/c2cc35084j.
Two new dibenzocyclooctyne-thymidine monomers were incorporated into oligonucleotides and crosslinked to azide-labelled complementary strands across the DNA grooves. Equivalent reactions were successful using a (bicyclo[6.1.0]nonyne) alkyne. Oligonucleotides containing internal cyclooctyne and amino groups were simultaneously reacted with azides and NHS esters of different fluorescent dyes to produce functional genetic probes.
两个新的二苯并环辛炔-胸腺嘧啶单体被引入寡核苷酸中，并通过 DNA 沟与叠氮标记的互补链交联。使用（双环[6.1.0]壬炔）炔烃可以成功进行等效反应。含有内部环辛炔和氨基的寡核苷酸与不同荧光染料的叠氮化物和 NHS 酯同时反应，生成功能遗传探针。
