Kojima Mikiko, Sakakibara Hitoshi
RIKEN Plant Science Center, Tsurumi, Yokohama, Japan.
Methods Mol Biol. 2012;918:151-64. doi: 10.1007/978-1-61779-995-2_11.
We describe a method for highly sensitive high-throughput analysis of six major phytohormones; cytokinins (23 species), auxins (7 species), abscisic acid (ABA), gibberellins (GAs, 11 species), salicylic acid (SA), and jasmonic acid (JA). The method consists of solid-phase extraction using 96-well column plates and liquid chromatography coupled with a tandem quadrupole mass spectrometer. In order to improve the quantification limit of negatively charged compounds and especially GAs, we use a chemical modification with an "MS-probe" that contains a quaternary amine moiety. The method requires plant tissue of <100 mg fresh weight for quantification and enables us to analyze large number of plant samples at a time.
我们描述了一种用于六种主要植物激素的高灵敏度高通量分析方法；细胞分裂素（23种）、生长素（7种）、脱落酸（ABA）、赤霉素（GAs，11种）、水杨酸（SA）和茉莉酸（JA）。该方法包括使用96孔柱板进行固相萃取以及液相色谱与串联四极杆质谱联用。为了提高带负电荷化合物尤其是GAs的定量限，我们使用了一种带有季铵基团的“质谱探针”进行化学修饰。该方法定量所需植物组织鲜重小于100mg，并且能够让我们一次分析大量植物样品。
