Sell Henrike, Jensen Jørgen, Eckel Juergen
Paul-Langerhans-Group Integrative Physiology, German Diabetes Center, Düsseldorf, Germany.
Methods Mol Biol. 2012;933:255-63. doi: 10.1007/978-1-62703-068-7_16.
Glucose disposal in skeletal muscle is a major target for insulin action and assessment of insulin-regulated glucose uptake under in vitro conditions allows the direct determination of insulin sensitivity in this organ. For this purpose, a variety of muscle preparations from different parts of the body can be used. We describe here a detailed protocol for using epitrochlearis muscle strips and additionally for using primary skeletal muscle cells.
